Detection of Human NTH1 by Western Blot.
Western blot shows lysates of DU145 human prostate carcinoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human NTH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2675) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NTH1 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
NTH1 (Endonuclease III) is a DNA glycosylase with AP lyase activity. It functions as an enzyme to repair oxidative damage to DNA through the BER (base excision repair) pathway.
Nth Endonuclease III-like 1
Entrez Gene IDs:
EC 18.104.22.168; endonuclease III-like protein 1; nth endonuclease III-like 1 (E. coli); NTH1; NTHL1; OCTS3; OCTS3NTH1nth (E.coli endonuclease III)-like 1
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