Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all OCIL/CLEC2d Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human OCIL/CLEC2d
Ser57-Val191
Accession # Q9UHP7

Specificity

Detects human OCIL/CLEC2d in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse (rm) OCIL is observed and less than 1% cross-reactivity with recombinant human (rh) CLEC2B, and rhCLEC2A is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human OCIL/CLEC2d Antibody

Detection of OCIL/CLEC2d antibody in Raji Human Cell Line antibody by Flow Cytometry.

Detection of OCIL/CLEC2d in Raji Human Cell Line by Flow Cytometry.

Raji human Burkitt's lymphoma cell line was stained with Goat Anti-Human OCIL/CLEC2d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3480, filled histo­gram) or control antibody (Catalog # AB-108-C, open histo­gram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Detection of OCIL/CLEC2d by Immunohistochemistry

Detection of OCIL/CLEC2d by Immunohistochemistry

LLT1 is highly expressed in immune-privileged sites.Lectin-like transcript 1 (LLT1) and isotype control stainings of testes (A), brain (B) and placenta (C) using the anti-LLT1 2H7 mAb (1/500). Representative image of three independent experiments. 20× magnification. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28413611), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of OCIL/CLEC2d by Immunohistochemistry

Detection of OCIL/CLEC2d by Immunohistochemistry

LLT1 is highly expressed in immune-privileged sites.Lectin-like transcript 1 (LLT1) and isotype control stainings of testes (A), brain (B) and placenta (C) using the anti-LLT1 2H7 mAb (1/500). Representative image of three independent experiments. 20× magnification. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28413611), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of OCIL/CLEC2d by Immunohistochemistry

Detection of OCIL/CLEC2d by Immunohistochemistry

LLT1 is expressed on B cells and monocytes.Lectin-like transcript 1 (LLT1) (stained using the 2H7 mAb) and CD161 levels were measured by flow cytometry in monocytes (M) and lymphocytes (L). (A) The gating strategy, (B) representative and cumulative data for the expression of CD161 (blue) and LLT1 (green) compared to the isotype control (IC, red) (n=4). (C) Representative image of LLT1 staining in human tonsil tissue using the 2H7 anti-LLT1 antibody (10×; n=6), together with a representative FACS plot of LLT1 staining of purified tonsillar B cells with the 2H7 antibody. The GC population is highlighted (rectangle; n=8). (D) Immunofluorescent co-staining of LLT1 (red) and CD68 (green) in lung and liver (scale bar = 100μm). Representative of two independent experiments. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28413611), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of OCIL/CLEC2d by Immunohistochemistry

Detection of OCIL/CLEC2d by Immunohistochemistry

LLT1 is highly expressed in immune-privileged sites.Lectin-like transcript 1 (LLT1) and isotype control stainings of testes (A), brain (B) and placenta (C) using the anti-LLT1 2H7 mAb (1/500). Representative image of three independent experiments. 20× magnification. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28413611), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human OCIL/CLEC2d Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

2.5 µg/106 cells
Sample: Raji human Burkitt's lymphoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human OCIL/CLEC2d

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OCIL/CLEC2d

Human OCIL/CLEC2d, also known as Lectin-Like Transcript-1 (LLT-1), is a type II transmembrane protein belonging to the natural killer (NK) cell receptor group of the C-type lectin superfamily. It is found on hematopoietic cells, osteoblasts, and chondrocytes. By alternative splicing, at least three isoforms exist. Isoform 1 is synthesized as a 191 amino acid (aa) precursor with a C-terminal 132 aa extracellular domain (ECD) that contains the C-type lectin domain. The aa sequence of human OCIL isoform 1 ECD is 49% and 50% identical to the mouse and rat OCIL ECD, respectively. Human OCIL preferentially binds high molecular weight sulfated glycosaminoglycans and is a ligand for the single human NKR-P1A receptor (CD161). Human OCIL blocks osteoclast differentiation. Engagement of OCIL with CD161 on NK cells also inhibits NK cell-mediated cytotoxicity and IFN-gamma secretion.

Long Name

Osteoclast Inhibitory Lectin

Alternate Names

CLEC2D, LLT1

Entrez Gene IDs

29121 (Human); 93694 (Mouse)

Gene Symbol

CLEC2D

UniProt

Q9UHP7

Additional OCIL/CLEC2d Products

Product Documents for Human OCIL/CLEC2d Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human OCIL/CLEC2d Antibody

For research use only

Related Research Areas

C-type Lectin Family

Citations for Human OCIL/CLEC2d Antibody

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