Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all p70 S6 Kinase beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human p70 S6 Kinase beta
Ala2-Ser410
Accession # Q9UBS0

Specificity

Detects human p70 S6 Kinase beta in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human p70 S6 Kinase is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human p70 S6 Kinase  beta Antibody

Detection of Human p70 S6 Kinase beta antibody by Western Blot.

Detection of Human p70 S6 Kinase beta by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for p70 S6 Kinase beta at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
p70 S6 Kinase beta antibody in MCF-7 Human Cell Line by Immunocytochemistry (ICC).

p70 S6 Kinase beta in MCF‑7 Human Cell Line.

p70 S6 Kinase beta was detected in immersion fixed MCF-7 human breast cancer cell line using Goat Anti-Human p70 S6 Kinase beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2987) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human p70 S6 Kinase  beta Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed MCF‑7 human breast cancer cell line

Western Blot

1 µg/mL
Sample: MCF‑7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p70 S6 Kinase beta

P70S6K beta (p70 ribosomal S6 kinase beta-2; also RPS6KB2, S6K beta, S6K beta 2, S6K2 and STK14B) is a monomeric, 54 kDa member of the AGC family of Ser/Thr kinases. It is widely expressed, and found predominately in the nucleus. Although it was initially reported to phosphorylate the ribosomal S6 protein, p70S6K beta /S6K beta 2 is now known to also phosphorylate RafB (cell survival), hnRNP (cell proliferation), p300/CBP (transcription) and CDC42 (cytoskeleton stability). Human p70S6K beta /S6K beta 2 is 482 amino acids in length. It contains a protein kinase domain (aa 67-328), a C-terminal regulatory region (aa 329-399) that contains an NLS (aa 384-389), and a Pro-rich segment (aa 411-482) that likely interacts with SH3 domain-containing proteins. Phosphorylation/dephosphorylation events and sumoylation regulate p70S6K beta activity. In addition to the 54 kDa form, there is a 56-60 kDa isoform that possesses a 13 aa addition at the N-terminus, plus an isoform that shows a deletion of aa 267-270. Over aa 1-410, human p70S6K beta 2 shares 95% and 75% aa identity with mouse p70S6K beta 2and human p70S6K beta 1, respectively.

Alternate Names

KLS, p70-beta, RPS6KB2, STK14B

Entrez Gene IDs

6199 (Human); 58988 (Mouse); 361696 (Rat)

Gene Symbol

RPS6KB2

UniProt

Q9UBS0

Additional p70 S6 Kinase beta Products

Product Documents for Human p70 S6 Kinase  beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human p70 S6 Kinase  beta Antibody

For research use only

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Protocols

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