Human Pappalysin-1/PAPP-A Antibody

Catalog # Availability Size / Price Qty
AF2487
AF2487-SP
Human Pappalysin-1/PAPP-A Antibody in Data
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Product Details
Citations (3)
FAQs
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Reviews (1)

Human Pappalysin-1/PAPP-A Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pappalysin‑1/PAPP‑A in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human PAPP-A2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pappalysin‑1/PAPP‑A
Glu82-Asp1214
Accession # Q13219
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Pappalysin‑1/PAPP-A (Catalog # 2487-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Pappalysin-1/ PAPP-A antibody by Western Blot. View Larger

Detection of Human Pappalysin‑1/ PAPP‑A by Western Blot. Western blot shows lysate of human pregnant sera. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Pappalysin-1/PAPP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2487) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pappalysin-1/PAPP-A at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pappalysin-1/PAPP-A

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (2). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (3). Lack of IGFBP-4 cleavage in embryonic fibroblasts derived from PAPP-A knockout mice indicates that PAPP-A functions as a physiological IGFBP-4 protease (4). Three Lin12-Notch repeats (LNR) in the PAPP-A protein bind Ca2+ and are required for the cleavage of IGFBP-4, not IGFBP-5, by PAPP-A (5). The C-terminal LNR (residues 1476 to 1503) is not present in rhPAPP-A (residues 82 to 1214), which starts at the N-terminus of the mature chain and ends before the five Sushi (SCR) modules. As an active protease, rhPAPP-A cleaves IGFBP-5, which can be inhibited by 1,10-phenanthroline.

References
  1. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
  2. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
  3. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
  4. Conover, C.A. et al. (2004) Development 131:1187.
  5. Boldt, H.B. et al. (2004) J. Biol. Chem. 279:38525.
Long Name
Pregnancy-associated Plasma Protein 1
Entrez Gene IDs
5069 (Human); 18491 (Mouse)
Alternate Names
ASBABP2; DIPLA1; EC 3.4.24.79; IGFBP-4ase; IGFBP-4aseaspecific BCL2 ARE-binding protein 2; IGF-dependent IGFBP-4 protease; insulin-like growth factor-dependent IGF binding protein-4 protease; Insulin-like growth factor-dependent IGF-binding protein 4 protease; PAPA; PAPPA; PAPPA1; PAPP-A1; PAPP-Adifferentially placenta 1 expressed protein; Pappalysin1; Pappalysin-1; pregnacy-associated plasma protein A; Pregnancy-associated plasma protein A; pregnancy-associated plasma protein A, pappalysin 1

Product Datasheets

Citations for Human Pappalysin-1/PAPP-A Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
    Sci Rep, 2016;6(0):32382.
    Species: Human
    Sample Types: Whole Cells
    Applications: IHC
  2. Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse.
    Authors: Wang J, Qiu Q, Haider M, Bell M, Gruslin A, Christians JK
    J. Endocrinol., 2009;202(3):337-45.
    Species: Human
    Sample Types: Serum
    Applications: Western Blot
  3. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
    Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
    Mol. Hum. Reprod., 2008;14(10):595-602.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot

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Human Pappalysin-1/PAPP-A Antibody
By Anonymous on 08/04/2020
Application: WB Sample Tested: Cell Lysates Species: Human