Human Pappalysin-1/PAPP-A Antibody Summary
Accession # Q13219
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Pappalysin‑1/ PAPP‑A by Western Blot. Western blot shows lysate of human pregnant sera. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Pappalysin-1/PAPP-A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2487) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Pappalysin-1/PAPP-A at approximately 200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (2). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (3). Lack of IGFBP-4 cleavage in embryonic fibroblasts derived from PAPP-A knockout mice indicates that PAPP-A functions as a physiological IGFBP-4 protease (4). Three Lin12-Notch repeats (LNR) in the PAPP-A protein bind Ca2+ and are required for the cleavage of IGFBP-4, not IGFBP-5, by PAPP-A (5). The C-terminal LNR (residues 1476 to 1503) is not present in rhPAPP-A (residues 82 to 1214), which starts at the N-terminus of the mature chain and ends before the five Sushi (SCR) modules. As an active protease, rhPAPP-A cleaves IGFBP-5, which can be inhibited by 1,10-phenanthroline.
- Boldt, H.B. et al. (2001) Biochem. J. 358:359.
- Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
- Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
- Conover, C.A. et al. (2004) Development 131:1187.
- Boldt, H.B. et al. (2004) J. Biol. Chem. 279:38525.
Citations for Human Pappalysin-1/PAPP-A Antibody
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Citations: Showing 1 - 3
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Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease
Sci Rep, 2016;6(0):32382.
Sample Types: Whole Cells
Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse.
Authors: Wang J, Qiu Q, Haider M, Bell M, Gruslin A, Christians JK
J. Endocrinol., 2009;202(3):337-45.
Sample Types: Serum
Applications: Western Blot
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
Mol. Hum. Reprod., 2008;14(10):595-602.
Sample Types: Tissue Homogenates
Applications: Western Blot
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