Human Pappalysin-2/PAPP-A2 Antibody

Catalog # Availability Size / Price Qty
AF1668
AF1668-SP
Product Details
Citations (3)
FAQs
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Human Pappalysin-2/PAPP-A2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Pappalysin‑2/PAPP‑A2 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human PAPP-A is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human Pappalysin‑2/PAPP‑A2
Ser234-Cys1396
Accession # Q9BXP8
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Pappalysin‑2/PAPP-A2 (Catalog # 1668-ZN)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Pappalysin‑2/PAPP-A2 (Catalog # 1668-ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Pappalysin-2/PAPP-A2

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP-E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5.

References
  1. Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
  2. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
  3. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
  4. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
Long Name
Pregnancy-associated Plasma Protein
Entrez Gene IDs
60676 (Human)
Alternate Names
EC 3.4.24.-; PAPPA2; PAPP-A2; PAPP-A2pappalysin-2; pappalysin 2; Pappalysin2; Pappalysin-2; PAPPE; PAPP-E; PLAC3; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; Pregnancy-associated plasma protein A2; Pregnancy-associated plasma protein E1

Product Datasheets

Citations for Human Pappalysin-2/PAPP-A2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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  1. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
    Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
    Reprod Biol Endocrinol, 2015;12(0):123.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Paraffin-embedded
  2. Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse.
    Authors: Wang J, Qiu Q, Haider M, Bell M, Gruslin A, Christians JK
    J. Endocrinol., 2009;202(3):337-45.
    Species: Human
    Sample Types: Serum
    Applications: WB
  3. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
    Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
    Mol. Hum. Reprod., 2008;14(10):595-602.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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