Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Transgenic Mouse

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA Development

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pappalysin-2/PAPP-A2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Pappalysin‑2/PAPP‑A2
Ser234-Cys1396
Accession # Q9BXP8

Specificity

Detects human Pappalysin‑2/PAPP‑A2 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human PAPP-A is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Pappalysin‑2/PAPP‑A2 Antibody

Detection of Pappalysin-2/PAPP-A2 by Western Blot

Detection of Pappalysin-2/PAPP-A2 by Western Blot

Effects of gene disruption on PAPP-A2 protein.Shown are Western blots of 9 representative (A) placentae and (B) embryos at embryonic day 12.5 from embryos homozygous for wild-type Pappa2 (+/+), heterozygous (+/−) or homozygous for the disruption (−/−). The nitrocellulose membrane was scanned for fluorescence at 700 and 800 nm simultaneously, with fluorescence at 700 nm corresponding to actin (at approximately 40 kDa) and fluorescence at 800 nm corresponding to PAPP-A2 (at approximately 250 kDa). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/23457539), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Pappalysin‑2/PAPP‑A2 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Pappalysin‑2/PAPP-A2 (Catalog # 1668-ZN), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Pappalysin‑2/PAPP-A2 (Catalog # 1668-ZN)

Reviewed Applications

Read 1 review rated 5 using AF1668 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pappalysin-2/PAPP-A2

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP-E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5.

References

  1. Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
  2. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
  3. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
  4. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.

Long Name

Pregnancy-associated Plasma Protein

Alternate Names

PAPP-A2, PAPPA2, Pappalysin2, PAPPE, PLAC3

Entrez Gene IDs

60676 (Human)

Gene Symbol

PAPPA2

UniProt

Q9BXP8

Additional Pappalysin-2/PAPP-A2 Products

Product Documents for Human Pappalysin‑2/PAPP‑A2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Pappalysin‑2/PAPP‑A2 Antibody

For research use only

Citations for Human Pappalysin‑2/PAPP‑A2 Antibody

Customer Reviews for Human Pappalysin‑2/PAPP‑A2 Antibody (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 23457539
    Species: Mouse
    Verified Customer | Posted 01/05/2015

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