Human Pappalysin-2/PAPP-A2 Antibody AF1668: R&D Systems

Human Pappalysin-2/PAPP-A2 Antibody

(3 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human Pappalysin‑2/PAPP‑A2 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human PAPP-A is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Pappalysin‑2/PAPP‑A2
    Ser234-Cys1396
    Accession # Q9BXP8
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Pappalysin-2/PAPP-A2

Pappalysins belong to a fifth family of metzincins that consists of ADAMs/ADAMTSs, MMPs, astacins and serrylysins (1, 2). PAPP-A is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy as compared to the nonpregnant state. PAPP-A is also a major marker of Down syndrome in the first trimester of pregnancy because maternal serum levels of PAPP-A are significantly reduced when a fetus affected by Down syndrome is present (3). PAPP-A cleaves Insulin-like Growth Factor-Binding Protein-4 and -5 (IGFBP-4 and -5) at a single site, resulting in the release of bioactive IGF (4). Compared to PAPP‑A, PAPP-A2 (also called PAPP-E), the second member of the family is less characterized. PAPP-A2 shares 45% amino acid identity to PAPP-A in the mature form, which is synthesized as a preproprotein consisting of multiple domains (1). The prepro region (residues 1‑233) and the C-terminal region (residues 1397‑1791) are not included in recombinant human PAPP-A2. As an active protease, recombinant human PAPP-A2 cleaves IGFBP-5.

  • References:
    1. Overgaard, M.T. et al. (2001) J. Biol. Chem. 276:21849.
    2. Boldt, H.B. et al. (2001) Biochem. J. 358:359.
    3. Fialova L. and I.M. Malbohan (2002) Bratisl. Lek. Listy 103:194.
    4. Laursen, L.S. et al. (2001) FEBS Lett. 504:36.
  • Long Name:
    Pregnancy-associated Plasma Protein
  • Entrez Gene IDs:
    60676 (Human)
  • Alternate Names:
    EC 3.4.24.-; PAPPA2; PAPP-A2; PAPP-A2pappalysin-2; pappalysin 2; Pappalysin2; Pappalysin-2; PAPPE; PAPP-E; PLAC3; placenta-specific 3; pregnancy-associated plasma preproprotein-A2; Pregnancy-associated plasma protein A2; Pregnancy-associated plasma protein E1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. IGFBP-4 and -5 are expressed in first-trimester villi and differentially regulate the migration of HTR-8/SVneo cells.
    Authors: Crosley, Erin J, Dunk, Caroline, Beristain, Alexande, Christians, Julian K
    Reprod Biol Endocrinol, 2015;12(0):123.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  2. Expression of pregnancy-associated plasma protein A2 during pregnancy in human and mouse.
    Authors: Wang J, Qiu Q, Haider M, Bell M, Gruslin A, Christians JK
    J. Endocrinol., 2009;202(3):337-45.
    Species: Human
    Sample Type: Serum
    Application: WB
  3. Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
    Authors: Nishizawa H, Pryor-Koishi K, Suzuki M
    Mol. Hum. Reprod., 2008;14(10):595-602.
    Species: Human
    Sample Type: Serum
    Application: ELISA Development
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