Human Parkin Antibody

Catalog # Availability Size / Price Qty
Detection of Human Parkin by Western Blot.
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Product Details
Supplemental Products

Human Parkin Antibody Summary

Species Reactivity
Detects human Parkin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Parkin-2 is observed.
Monoclonal Mouse IgG2B Clone # 323302
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Parkin
Accession # O60260
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

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Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Parkin antibody by Western Blot. View Larger

Detection of Human Parkin by Western Blot. Western blot shows lysates ofSf21S. frugiperdainsect ovarian cell line mock transfected or transfected with GST tagged Parkin. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Parkin Monoclonal Antibody (Catalog # MAB14381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GST tagged Parkin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Parkin

Parkin is a 465 amino acid protein with an N-terminal ubiquitin-like domain, a linker region, a C-terminal TRIAD domain consisting of two RING finger motifs flanking a cysteine-rich IBR (in between RING fingers) motif. Mutations in Parkin are a major cause of autosomal recessive juvenile parkinsonism. Parkin-2 lacks exon 5, which encodes aa 179‑206 of Parkin.

Long Name
Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1
Entrez Gene IDs
5071 (Human); 50873 (Mouse); 56816 (Rat)
Alternate Names
AR-JP; E3 ubiquitin ligase; E3 ubiquitin-protein ligase parkin; EC 6.3.2.-; LPRS2; PARK2; parkin 2; Parkin; Parkinson disease protein 2; Parkinson juvenile disease protein 2; parkinson protein 2, E3 ubiquitin protein ligase (parkin); PDJ; PDJjuvenile) 2, parkin; PRKN

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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