Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 323302
View all Parkin Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Parkin
Met1-Val465
Accession # O60260

Specificity

Detects human Parkin in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Parkin-2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Parkin Antibody

Detection of Human Parkin antibody by Western Blot.

Detection of Human Parkin by Western Blot.

Western blot shows lysates ofSf21S. frugiperdainsect ovarian cell line mock transfected or transfected with GST tagged Parkin. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Parkin Monoclonal Antibody (Catalog # MAB14381) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GST tagged Parkin at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Parkin Antibody

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Sf 21 S. frugiperda insect ovarian cell line transfected GST tagged Parkin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Parkin

Parkin is a 465 amino acid protein with an N-terminal ubiquitin-like domain, a linker region, a C-terminal TRIAD domain consisting of two RING finger motifs flanking a cysteine-rich IBR (in between RING fingers) motif. Mutations in Parkin are a major cause of autosomal recessive juvenile parkinsonism. Parkin-2 lacks exon 5, which encodes aa 179‑206 of Parkin.

Long Name

Parkinson Disease [autosomal recessive, juvenile] 2, Parkin [PARK2], transcript variant 1

Alternate Names

AR-JP, PARK2, PDJ, PRKN

Entrez Gene IDs

5071 (Human); 50873 (Mouse); 56816 (Rat)

Gene Symbol

PRKN

UniProt

O60260 (Human)

Additional Parkin Products

Product Documents for Human Parkin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Parkin Antibody

For research use only

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