Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Avian - Chicken

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Pax3/Pax7 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pax3
Met1-Ser215
Accession # NP_000429

Specificity

Detects Pax3/Pax7 in direct ELISAs and Western blots. In Western blots, approximately 2% cross-reactivity with recombinant human (rh) Pax5, under reducing conditions, is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Pax3/Pax7 Antibody

Detection of Pax3/Pax7 in Human Skin.

Detection of Pax3/Pax7 in Human Skin.

Pax3 was detected in immersion fixed paraffin-embedded sections of human skin using Goat Anti-Human Pax3/Pax7 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2457) at 3 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004) or the HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the nucleus of melanocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human Pax3/Pax7 Antibody

Application
Recommended Usage

Immunohistochemistry

3 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human skin

Western Blot

0.1 µg/mL
Sample: Recombinant Human Pax3

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Pax3/Pax7

Pax3 belongs to the family of paired box transcription factors. Pax family proteins typically contain a paired box domain and a paired-type homeodomain. These transcription factors play critical roles during fetal development. There is 98% homology between human and mouse Pax3.

Long Name

Paired Box Gene 3 /Paired Box Gene 7

UniProt

NP_000429

Additional Pax3/Pax7 Products

Product Documents for Human Pax3/Pax7 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Pax3/Pax7 Antibody

For research use only

Citations for Human Pax3/Pax7 Antibody

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Protocols

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