Human PBEF/Visfatin DuoSet ELISA

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Human PBEF / Visfatin ELISA Standard Curve
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Product Details
Citations (7)
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Human PBEF/Visfatin DuoSet ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
100 µL
Assay Range
0.5 - 32 ng/mL
Sufficient Materials
For five 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

Product Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits

Kit Content

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

Human PBEF / Visfatin ELISA Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PBEF/Visfatin

PBEF, also called Nampt or visfatin, is a ubiquitous 52 kDa nicotinamide phosphoribosyltransferase. PBEF is the rate-limiting component in the biosynthesis of NAD+, and functions in the cytoplasm to regulate energy metabolism during stress responses and immune activation. Although it lacks a signal sequence, PBEF appears to be secreted by visceral adipose and functions as a noncompetitive insulin mimetic. Mouse PBEF shows 96% and > 99% aa identity with human and rat PBEF, respectively.

Long Name:
Pre-B-Cell Colony-Enhancing Factor 1
Entrez Gene IDs:
10135 (Human); 59027 (Mouse); 297508 (Rat)
Alternate Names:
EC; MGC117256; NAmPRTase; NAMPT; nicotinamide phosphoribosyltransferase; NMPRTase; PBEF; PBEF1; PBEF1110035O14Rik; PBEF1DKFZp666B131; Pre-B cell-enhancing factor; pre-B-cell colony enhancing factor 1; Pre-B-cell colony-enhancing factor 1; VF; Visfatin

Citations for Human PBEF/Visfatin DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. Extracellular Nicotinamide Phosphoribosyltransferase as a Surrogate Marker of Prominent Malignant Potential in Colonic Polyps: A 2-Year Prospective Study
    Authors: TH Chen, HC Hsu, JF You, CC Lai, YK Tsou, CL Hsu, CSJ Fann, RN Chien, ML Chang
    Cancers, 2023-03-10;15(6):.
    Species: Human
    Sample Types: Plasma
  2. High Glucose Promotes Inflammation and Weakens Placental Defenses against E. coli and S. agalactiae Infection: Protective Role of Insulin and Metformin
    Authors: R Jiménez-Es, D Vargas-Alc, P Flores-Esp, AC Helguera-R, O Villavicen, I Mancilla-H, C Irles, YD Torres-Ram, MY Valdespino, P Velázquez-, R Zamora-Esc, M Islas-Lópe, C Carranco-S, L Díaz, V Zaga-Clave, A Olmos-Orti
    International Journal of Molecular Sciences, 2023-03-09;24(6):.
    Species: Human
    Sample Types: Placental Supernates
  3. Visfatin Enhances Breast Cancer Progression through CXCL1 Induction in Tumor-Associated Macrophages
    Authors: YY Wang, HD Chen, S Lo, YK Chen, YC Huang, SC Hu, YC Hsieh, AC Hung, MF Hou, SF Yuan
    Cancers, 2020-11-26;12(12):.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Expression of adipokines in seminal fluid of men of normal weight
    Authors: A Bongrani, Y Elfassy, JS Brun, C Ramé, N Mellouk, S Fellahi, JP Bastard, R Levy, C Vasseur, P Froment, J Dupont
    Asian J. Androl., 2019-09-01;0(0):.
    Species: Human
    Sample Types: Seminal Fluid
  5. ?-Mangostin Alleviated Lipopolysaccharide Induced Acute Lung Injury in Rats by Suppressing NAMPT/NAD Controlled Inflammatory Reactions
    Authors: M Tao, J Jiang, L Wang, Y Li, Q Mao, J Dong, J Zuo
    Evid Based Complement Alternat Med, 2018-10-10;2018(0):5470187.
    Species: Rat
    Sample Types: Serum
  6. Nesfatin-1 and visfatin expression is associated with reduced atherosclerotic disease risk in patients with rheumatoid arthritis
    Authors: C Robinson, L Tsang, A Solomon, AJ Woodiwiss, S Gunter, M Mer, HC Hsu, M Gomes, GR Norton, AME Millen, PH Dessein
    Peptides, 2018-02-21;102(0):31-37.
    Species: Human
    Sample Types: Plasma
  7. Presence of IL-17 in synovial fluid identifies a potential inflammatory osteoarthritic phenotype
    Authors: SJ Snelling, S Bas, GJ Puskas, SG Dakin, D Suva, A Finckh, C Gabay, P Hoffmeyer, AJ Carr, A Lübbeke
    PLoS ONE, 2017-04-11;12(4):e0175109.
    Species: Human
    Sample Types: Serum


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