This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human PD-ECGF / Thymidine Phosphorylase. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Store the unopened product at 2 - 8 degreesC. Do not use past expiration date.
Background: PD-ECGF/Thymidine Phosphorylase
PD-ECGF was originally isolated as a platelet-derived endothelial cell mitogen (based on its ability to stimulate 3H-thymidine incorporation) and its cDNA was cloned from a human placental cDNA library. In solution, PD-ECGF exists as a non-disulfide linked homodimer. The sequence of human PD-ECGF is identical to that of human thymidine phosphorylase (dThdPase).
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