Solid Phase Sandwich ELISA
96-well strip plate
Sample Type & Volumne Required
15.60 - 1,000 pg/mL
For fifteen 96-well plates*
- * Following cell simulation.
* Provided that the recommended microplates, buffers, diluents, substrates and solutions
are used, and the assay is run as summarized in the Assay Procedure provided.
This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and Recombinant . The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet.
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
(Catalog # DY006
), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2
, 1.5 mM KH2
, pH 7.2 - 7.4, 0.2 µm filtered Wash Buffer:
(Catalog # WA126
), or equivalent Reagent Diluent* Blocking Buffer* Substrate Solution:
1:1 mixture of Color Reagent A (H2
) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999
) Stop Solution:
2 N H2
(Catalog # DY994
: R&D Systems (Catalog # DY990
), or equivalent Plate Sealers:
ELISA Plate Sealers (Catalog # DY992
), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .
The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
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