Human PDGF-B Antibody

Catalog # Availability Size / Price Qty
MAB1739-SP
MAB1739-500
MAB1739-100
Product Details
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Human PDGF-B Antibody Summary

Species Reactivity
Human
Specificity
Detects the human PDGF-B subunit in direct ELISAs and Western blots. In Western blots, shows 50-100% cross-reactivity with recombinant human (rh) PDGF‑AB and rrPDGF-BB but no cross-reactivity with rhCTGF, rhPlGF, rhVEGF‑B186, rhVEGF‑C, or rhVEGF‑D.
Source
Monoclonal Mouse IgG1 Clone # 108128
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human PDGF-B (Catalog # 220-BB)
under non-reducing conditions only

Human PDGF-AB Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human PDGF-AA/AB Biotinylated Antibody (Catalog # BAF221)

Standard: Recombinant Human PDGF-AB Protein, CF (Catalog # 222-AB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-B

Four PDGF family members, A, B, C and D, have been identified. PDGF-B can exist as a disulfide-linked homodimer (PDGF-BB) or heterodimer (PDGF-AB). PDGF-B binds and activates PDGF R alpha and PDGF R beta.

Long Name
Platelet-derived Growth Factor B
Entrez Gene IDs
5155 (Human); 24628 (Rat); 100070283 (Equine)
Alternate Names
B chain; Becaplermin; C-Sis; FLJ12858; IBGC5; PDGF subunit B; PDGF2; PDGF-2; PDGFB; PDGF-B; platelet-derived growth factor 2; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog); Platelet-derived growth factor beta polypeptide; platelet-derived growth factor subunit B; platelet-derived growth factor, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis; SIS; SSV

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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Human PDGF-B Antibody
By Anonymous on 07/19/2019
Application: ELISA Sample Tested: Serum and Plasma Species: Human