Human PDGF‑D Antibody

Detection of Mouse PDGF-D/SCDGFB by Western Blot

RPE-specific overexpression of PDGF-D in vivo.(A) Schematic diagram illustrating the AAV vector carrying GFP (AAV-GFP) or human PDGF-D (AAV-PDGF-D) gene and RPE specific VDM2 promoter. (B) Real-time PCR results of relative mRNA expression of PDGF-D in retinae or RPE-choroid complex from mice injected with AAV-GFP or AAV-PDGF- D for 4 weeks. (C) Representative immunoblotting showing PDGF-D expression in mouse retinae or RPE-choroid complex. (D) Quantifications of PDGF-D protein levels in (C). (E) Immunofluorescence images highlighting RPE-specific expression of GFP or PDGF-D in mouse RPE-choroid complex with 4 weeks AAV-GFP or AAV-PDGF-D injection, respectively. Scale bar: 50 μm. n = 5, ****p < 0.0001, ns: not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/34150781), licensed under a CC-BY license. Not internally tested by R&D Systems.