Human PEG10 Antibody

Catalog # Availability Size / Price Qty
MAB99751-100
MAB99751-SP
Detection  of PEG-10 in Human HepG2 cell line by Flow Cytometry.
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Human PEG10 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PEG10 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 981902
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Synthetic peptide containing human PEG10
Accession # Q86TG7
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human PEG10 Monoclonal Antibody (Catalog # MAB9975).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of PEG-10 in Human HepG2 cell line by Flow Cytometry. View Larger

Detection of PEG-10 in Human HepG2 cell line by Flow Cytometry. HepG2 human hepatoma cell line was stained with Mouse Anti-Human PEG-10 Monoclonal Antibody (Catalog # MAB99751, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody(Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PEG10

Paternally Expressed Gene-10, or PEG10 (a.k.a Retrotransposon-derived protein PEG10, EDR, MART2, MEF3L1 and RGAG3) is a 708 amino acids (aa) protein encoded by the PEG10 gene. PEG-10 Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1, possibly playing a key role in epithelial-mesenchymal transition (EMT). PEG-10 has been suggested to participate in cell proliferation, differentiation and cell apoptosis processes and identified as a prognostic biomarker for progression of several carcinomas, like breast, lung, liver and pancreatic carcinomas.

Long Name
Paternally Expressed 10
Entrez Gene IDs
23089 (Human); 170676 (Mouse); 678843 (Rat)
Alternate Names
EDR; embryonal carcinoma differentiation regulated; Embryonal carcinoma differentiation-regulated protein; HB-1; KIAA1051EDR; Mammalian retrotransposon-derived protein 2; Mar2; Mart2; MEF3 Like 1; MEF3L; MEF3L1; Myelin expression factor 3-like protein 1; paternally expressed 10; Paternally expressed gene 10 protein; PEG10; retrotransposon gag domain containing 3; Retrotransposon gag domain-containing protein 3; Retrotransposon-derived gag-like polyprotein; retrotransposon-derived protein PEG10; RGAG3; RGAG3MEF3-like protein 1; RTL2; SIRH1; Ty3/Gypsy-like protein

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

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Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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