Key Product Details

Species Reactivity

Human

Applications

ELISA, Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 981902
View all PEG10 Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide containing human PEG10
Accession # Q86TG7

Specificity

Detects human PEG10 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human PEG10 Antibody

Detection of PEG-10 antibody in Human HepG2 cell line antibody by Flow Cytometry.

Detection of PEG-10 in Human HepG2 cell line by Flow Cytometry.

HepG2 human hepatoma cell line was stained with Mouse Anti-Human PEG-10 Monoclonal Antibody (Catalog # MAB99751, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by PE-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Applications for Human PEG10 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human PEG10 Monoclonal Antibody (Catalog # MAB9975).

This product is intended for assay development on various assay platforms requiring antibody pairs.

Intracellular Staining by Flow Cytometry

0.25 µg/106 cells
Sample: HepG2 Human Cell line fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012)

Flow Cytometry Panel Builder

Bio-Techne Knows Flow Cytometry

Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
Build Your Panel Now
Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PEG10

Paternally Expressed Gene-10, or PEG10 (a.k.a Retrotransposon-derived protein PEG10, EDR, MART2, MEF3L1 and RGAG3) is a 708 amino acids (aa) protein encoded by the PEG10 gene. PEG-10 Inhibits the TGF-beta signaling by interacting with the TGF-beta receptor ALK1, possibly playing a key role in epithelial-mesenchymal transition (EMT). PEG-10 has been suggested to participate in cell proliferation, differentiation and cell apoptosis processes and identified as a prognostic biomarker for progression of several carcinomas, like breast, lung, liver and pancreatic carcinomas.

Long Name

Paternally Expressed 10

Alternate Names

EDR, HB-1, Mar2, MEF3 Like 1, MEF3L, MEF3L1, RGAG3, RTL2, SIRH1

Entrez Gene IDs

23089 (Human); 170676 (Mouse); 678843 (Rat)

Gene Symbol

PEG10

UniProt

Q86TG7

Additional PEG10 Products

Product Documents for Human PEG10 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PEG10 Antibody

For research use only

Customer Reviews for Human PEG10 Antibody

There are currently no reviews for this product. Be the first to review Human PEG10 Antibody and earn rewards!

Have you used Human PEG10 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies