This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Peroxiredoxin 2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.
Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Peroxiredoxin 2
Peroxiredoxins make up a family of highly conserved peroxidases well known for their anti-oxidant properties. In this capacity they may be involved in the protection of cells from oxidative stress. They have roles as modulators of signal transduction, transcription, and apoptosis. To date, 6 family members have been described in mammals (peroxiredoxin 1 through 6). Peroxiredoxins are further subclassified based on the position of cysteine residues that are involved in catalysis (1-Cys, 2-Cys, and atypical 2-Cys).
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.