Human Peroxiredoxin 2 DuoSet ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
- Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Economical alternative to complete kits
- Capture Antibody
- Detection Antibody
- Recombinant Standard
- Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Human Peroxiredoxin 2 ELISA Standard Curve
Preparation and Storage
Background: Peroxiredoxin 2
Peroxiredoxins make up a family of highly conserved peroxidases well known for their anti-oxidant properties. In this capacity they may be involved in the protection of cells from oxidative stress. They have roles as modulators of signal transduction, transcription, and apoptosis. To date, 6 family members have been described in mammals (peroxiredoxin 1 through 6). Peroxiredoxins are further subclassified based on the position of cysteine residues that are involved in catalysis (1-Cys, 2-Cys, and atypical 2-Cys).
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