Human Phospho-eIF4B (S422) Antibody

Catalog # Availability Size / Price Qty
MAB3800
MAB3800-SP
Detection of Human Phospho-eIF4B (S422) by Western Blot.
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Human Phospho-eIF4B (S422) Antibody Summary

Species Reactivity
Human
Specificity
Detects human eIF4B when phosphorylated at S422
Source
Monoclonal Rat IgG2A Clone # 866049
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Phosphopeptide containing the human eIF4B S422 site
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Phospho-eIF4B (S422) by Western Blot. View Larger

Detection of Human Phospho-eIF4B (S422) by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes or 100 ng/mL Recombinant Human EGF (Catalog # 236-EG) for 5 minutes. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human Phospho-eIF4B (S422) Monoclonal Antibody (Catalog # MAB3800) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Phospho-eIF4B (S422) at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: eIF4B

eIF4B (eukaryotic translation initiation factor 4B), an RNA binding protein, is essential for the binding of mRNA to the 43S pre-initiation complex, which consists of the 40S ribosomal subunit bound to a ternary complex of eIF2, GTP, and Met-tRNA stabilized by eIF3. An arginine rich motif (ARM) in the carboxy-terminus of eIF4B binds RNA non-specifically, while a canonical RNA motif (RMM) near the amino terminus binds specifically to 18S rRNA. The simultaneous binding of specific and nonspecific RNA may serve to facilitate the binding of the 40S subunit to the mRNA by serving as a bridge between the 18S rRNA and the mRNA. Besides its binding activity, eIF4B in conjunction with the heterotrimeric eIF4F, stimulates the ATPase and RNA helicase activity of eIF4E.

Long Name
Eukaryotic Translation Initiation Factor 4B
Entrez Gene IDs
1975 (Human); 75705 (Mouse); 300253 (Rat)
Alternate Names
Eif4a2; eIF4B; EIF-4B; eukaryotic translation initiation factor 4B; PRO1843

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