Human Phospho-ErbB2/Her2 DuoSet IC ELISA
Human Phospho-ErbB2/Her2 DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15-(96-well) plate pack sizes
- Economical alternative to Western blot
Kit Content
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Product Datasheets
Preparation and Storage
Background: ErbB2/Her2
ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.
Citations for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
8
Citations: Showing 1 - 8
Filter your results:
Filter by:
-
Andrographolide enhanced 5-fluorouracil-induced antitumor effect in colorectal cancer via inhibition of c-MET pathway
Authors: M Su, B Qin, F Liu, Y Chen, R Zhang
Drug Des Devel Ther, 2017;11(0):3333-3341.
Species: Human
Sample Types: Cell Culture Supernates
-
Design, synthesis, docking and QSAR study of substituted benzimidazole linked oxadiazole as cytotoxic agents, EGFR and erbB2 receptor inhibitors
Authors: Md Jawaid Akhtar
Eur J Med Chem, 2016;126(0):853-869.
Species: Human
Sample Types: Cell Culture Supernates
-
Cellular Effects of HER3-Specific Affibody Molecules.
Authors: Gostring L, Malm M, Hoiden-Guthenberg I, Frejd FY, Stahl S, Lofblom J, Gedda L
PLoS ONE, 2012;7(6):e40023.
Species: Human
Sample Types: Cell Lysates
-
The HER2-binding affibody molecule (Z(HER2ratio342))(2) increases radiosensitivity in SKBR-3 cells.
Authors: Ekerljung L, Lennartsson J, Gedda L
PLoS ONE, 2012;7(11):e49579.
Species: Human
Sample Types: Cell Lysates
-
Combined vaccination with HER-2 peptide followed by therapy with VEGF peptide mimics exerts effective anti-tumor and anti-angiogenic effects in vitro and in vivo.
Authors: Foy, Kevin C, Miller, Megan J, Moldovan, Nicanor, Carson, William, Kaumaya, Pravin T
Oncoimmunology, 2012;1(7):1048-1060.
Species: Human
Sample Types: Cell Culture Supernates
-
Site-specific coupling and sterically controlled formation of multimeric antibody fab fragments with unnatural amino acids.
Authors: Hutchins BM, Kazane SA, Staflin K, Forsyth JS, Felding-Habermann B, Schultz PG, Smider VV
J. Mol. Biol., 2011;406(4):595-603.
Species: Human
Sample Types: Cell Lysates
-
AZD8931, an equipotent, reversible inhibitor of signaling by epidermal growth factor receptor, ERBB2 (HER2), and ERBB3: a unique agent for simultaneous ERBB receptor blockade in cancer.
Authors: Hickinson DM, Klinowska T, Speake G
Clin. Cancer Res., 2010;16(4):1159-69.
Species: Human
Sample Types: Cell Lysates
-
Peptide vaccines of the HER-2/neu dimerization loop are effective in inhibiting mammary tumor growth in vivo.
Authors: Allen SD, Garrett JT, Rawale SV, Jones AL, Phillips G, Forni G, Morris JC, Oshima RG, Kaumaya PT
J. Immunol., 2007;179(1):472-82.
Species: Human
Sample Types: Cell Lysates
FAQs
-
Does the capture antibody in the Human Phospho-ErbB2/Her2 DuoSet IC ELISA, Catalog # DYC1768, detect Erb2 or phosphorylated Erb2?
The capture antibody detects and binds both phosphorylated and unphosphorylated Erb2. The conjugated detection antibody specific for phosphorylated tyrosine will detect only the phosphorylated protein.
Supplemental ELISA Products
Reviews for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
There are currently no reviews for this product. Be the first to review Human Phospho-ErbB2/Her2 DuoSet IC ELISA and earn rewards!
Have you used Human Phospho-ErbB2/Her2 DuoSet IC ELISA?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
