Human Phospho-ErbB2/Her2 DuoSet IC ELISA

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Citations (8)
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Human Phospho-ErbB2/Her2 DuoSet IC ELISA Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Sample Volume Required
Cell lysates (100 µL)
Sufficient Materials
Kits available for two, five, or fifteen 96-well plates*
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet IC ELISA contains the basic components required for the development of sandwich ELISAs to measure tyrosine-phosphorylated human ErbB2 / Her2 in cell lysates. An immobilized capture antibody specific for ErbB2 / Her2 binds both phosphorylated and unphosphorylated ErbB2 / Her2. After washing away unbound material, a biotinylated detection antibody is used to detect only phosphorylated receptor, utilizing a standard HRP format.

Product Features

  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Available in 2, 5, and 15-(96-well) plate pack sizes
  • Economical alternative to Western blot

Kit Content

  • Capture Antibody
  • Conjugated Detection Antibody
  • Calibrated Immunoassay Standard or Control

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Lysis Buffer*

IC Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product

Product Datasheets

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Preparation and Storage

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ErbB2/Her2

ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.

Long Name:
Receptor Tyrosine Protein Kinase ErbB2
Entrez Gene IDs:
2064 (Human); 13866 (Mouse); 24337 (Rat); 102146608 (Cynomolgus Monkey)
Alternate Names:
CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomaderived oncogene homolog (avian)

Citations for Human Phospho-ErbB2/Her2 DuoSet IC ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Andrographolide enhanced 5-fluorouracil-induced antitumor effect in colorectal cancer via inhibition of c-MET pathway
    Authors: M Su, B Qin, F Liu, Y Chen, R Zhang
    Drug Des Devel Ther, 2017-11-23;11(0):3333-3341.
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Design, synthesis, docking and QSAR study of substituted benzimidazole linked oxadiazole as cytotoxic agents, EGFR and erbB2 receptor inhibitors
    Authors: Md Jawaid Akhtar
    Eur J Med Chem, 2016-12-09;126(0):853-869.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. The HER2-binding affibody molecule (Z(HER2ratio342))(2) increases radiosensitivity in SKBR-3 cells.
    Authors: Ekerljung L, Lennartsson J, Gedda L
    PLoS ONE, 2012-11-14;7(11):e49579.
    Species: Human
    Sample Types: Cell Lysates
  4. Combined vaccination with HER-2 peptide followed by therapy with VEGF peptide mimics exerts effective anti-tumor and anti-angiogenic effects in vitro and in vivo.
    Authors: Foy, Kevin C, Miller, Megan J, Moldovan, Nicanor, Carson, William, Kaumaya, Pravin T
    Oncoimmunology, 2012-10-01;1(7):1048-1060.
    Species: Human
    Sample Types: Cell Culture Supernates
  5. Cellular Effects of HER3-Specific Affibody Molecules.
    Authors: Gostring L, Malm M, Hoiden-Guthenberg I, Frejd FY, Stahl S, Lofblom J, Gedda L
    PLoS ONE, 2012-06-29;7(6):e40023.
    Species: Human
    Sample Types: Cell Lysates
  6. Site-specific coupling and sterically controlled formation of multimeric antibody fab fragments with unnatural amino acids.
    Authors: Hutchins BM, Kazane SA, Staflin K, Forsyth JS, Felding-Habermann B, Schultz PG, Smider VV
    J. Mol. Biol., 2011-01-13;406(4):595-603.
    Species: Human
    Sample Types: Cell Lysates
  7. AZD8931, an equipotent, reversible inhibitor of signaling by epidermal growth factor receptor, ERBB2 (HER2), and ERBB3: a unique agent for simultaneous ERBB receptor blockade in cancer.
    Authors: Hickinson DM, Klinowska T, Speake G
    Clin. Cancer Res., 2010-02-09;16(4):1159-69.
    Species: Human
    Sample Types: Cell Lysates
  8. Peptide vaccines of the HER-2/neu dimerization loop are effective in inhibiting mammary tumor growth in vivo.
    Authors: Allen SD, Garrett JT, Rawale SV, Jones AL, Phillips G, Forni G, Morris JC, Oshima RG, Kaumaya PT
    J. Immunol., 2007-07-01;179(1):472-82.
    Species: Human
    Sample Types: Cell Lysates


  1. Does the capture antibody in the Human Phospho-ErbB2/Her2 DuoSet IC ELISA, Catalog # DYC1768, detect Erb2 or phosphorylated Erb2?

    • The capture antibody detects and binds both phosphorylated and unphosphorylated Erb2. The conjugated detection antibody specific for phosphorylated tyrosine will detect only the phosphorylated protein. 

  2. Which phosphorylated sites are recognized by this assay?

    • This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.

  3. Does this assay detect both phosphorylated and non-phosphorylated Erb2?

    • The capture antibody binds both phosphorylated and non-phosphorylated Erb2. The conjugated detection antibody is specific for phosphorylated tyrosine so the assay will detect only phosphorylated Erb2.

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