ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.
Human Phospho-ErbB2/Her2 DuoSet IC ELISA
R&D Systems | Catalog # DYC1768-2
Key Product Details
Assay Type
Sample Type
Reactivity
Human Phospho-ErbB2/Her2 DuoSet IC ELISA Features
- Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15-(96-well) plate pack sizes
- Economical alternative to Western blot
Product Summary for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
Product Specifications
Assay Format
Sample Volume Required
Detection Method
Conjugate
Specificity
Label
Kit Contents for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Preparation and Storage
Shipping
Stability & Storage
Background: ErbB2/Her2
Long Name
Alternate Names
Gene Symbol
Additional ErbB2/Her2 Products
Product Documents for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
For research use only
Related Research Areas
Citations for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- ELISA Sample Preparation & Collection Guide
- ELISA Troubleshooting Guide
- How to Run an R&D Systems DuoSet ELISA
- How to Run an R&D Systems Quantikine ELISA
- How to Run an R&D Systems Quantikine™ QuicKit™ ELISA
- Quantikine HS ELISA Kit Assay Principle, Alkaline Phosphatase
- Quantikine HS ELISA Kit Principle, Streptavidin-HRP Polymer
- Sandwich ELISA (Colorimetric) – Biotin/Streptavidin Detection Protocol
- Sandwich ELISA (Colorimetric) – Direct Detection Protocol
- Troubleshooting Guide: ELISA
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
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Q: Does the capture antibody in the Human Phospho-ErbB2/Her2 DuoSet IC ELISA, Catalog # DYC1768, detect Erb2 or phosphorylated Erb2?
A: The capture antibody detects and binds both phosphorylated and unphosphorylated Erb2. The conjugated detection antibody specific for phosphorylated tyrosine will detect only the phosphorylated protein.
-
Q: Does this assay detect both phosphorylated and non-phosphorylated Erb2?
A: The capture antibody binds both phosphorylated and non-phosphorylated Erb2. The conjugated detection antibody is specific for phosphorylated tyrosine so the assay will detect only phosphorylated Erb2.
-
Q: Which phosphorylated sites are recognized by this assay?
A: This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
-
Q: Does the capture antibody in the Human Phospho-ErbB2/Her2 DuoSet IC ELISA, Catalog # DYC1768, detect Erb2 or phosphorylated Erb2?
A: The capture antibody detects and binds both phosphorylated and unphosphorylated Erb2. The conjugated detection antibody specific for phosphorylated tyrosine will detect only the phosphorylated protein.
-
Q: Does this assay detect both phosphorylated and non-phosphorylated Erb2?
A: The capture antibody binds both phosphorylated and non-phosphorylated Erb2. The conjugated detection antibody is specific for phosphorylated tyrosine so the assay will detect only phosphorylated Erb2.
-
Q: Which phosphorylated sites are recognized by this assay?
A: This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
-
Q: Does the capture antibody in the Human Phospho-ErbB2/Her2 DuoSet IC ELISA, Catalog # DYC1768, detect Erb2 or phosphorylated Erb2?
A: The capture antibody detects and binds both phosphorylated and unphosphorylated Erb2. The conjugated detection antibody specific for phosphorylated tyrosine will detect only the phosphorylated protein.
-
Q: Does this assay detect both phosphorylated and non-phosphorylated Erb2?
A: The capture antibody binds both phosphorylated and non-phosphorylated Erb2. The conjugated detection antibody is specific for phosphorylated tyrosine so the assay will detect only phosphorylated Erb2.
-
Q: Which phosphorylated sites are recognized by this assay?
A: This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
Associated Pathways
MAPK Signaling Pathway: Mitogen Stimulation Pathway
mTOR Signaling Pathway
