Human Phospho-ErbB2/Her2 DuoSet IC ELISA
Human Phospho-ErbB2/Her2 DuoSet IC ELISA Summary
* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.
Product Features
- Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
- Development protocols are provided to guide further assay optimization
- Assay can be customized to your specific needs
- Available in 2, 5, and 15-(96-well) plate pack sizes
- Economical alternative to Western blot
Kit Content
- Capture Antibody
- Conjugated Detection Antibody
- Calibrated Immunoassay Standard or Control
Other Reagents Required
PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2O4, pH 7.2 - 7.4, 0.2 µm filtered
Wash Buffer: (Catalog # WA126), or equivalent
Lysis Buffer*
IC Diluent*
Blocking Buffer*
Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)
Stop Solution: 2 N H2SO4 (Catalog # DY994)
Microplates: R&D Systems (Catalog # DY990), or equivalent
Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent
*For the Lysis Buffer, IC Diluent, and Blocking BUffer recommended for a specific DuoSet ELISA Development Kit, please see the product
Product Datasheets
Preparation and Storage
Background: ErbB2/Her2
ErbB2, also called Neu and Her2, is a transmembrane glycoprotein in the ErbB family of tyrosine kinase receptors for EGF superfamily growth factors. ErbB2 is widely expressed in epithelial cells and over-expressed in a large number of breast carcinomas. ErbB2 has no identified ligands but heterodimerizes with ErbB1/EGF R, ErbB3, or ErbB4 to form higher affinity signaling complexes. The protease ADAM10 releases a 110 kDa soluble fragment of ErbB2 from the cell surface. ErbB2 plays roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation. The ErbB2/ErbB3 heterodimer is expressed in the majority of breast, skin, ovary and gastrointestinal tumors and transduces a highly mitogenic signal in response to neuregulin 1 (NRG1; heuregulin 1) or NRG2. ErbB3, ErbB2 and neuregulin are all required for formation of the sympathetic nervous system.
Citations for Human Phospho-ErbB2/Her2 DuoSet IC ELISA
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 8
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Andrographolide enhanced 5-fluorouracil-induced antitumor effect in colorectal cancer via inhibition of c-MET pathway
Authors: M Su, B Qin, F Liu, Y Chen, R Zhang
Drug Des Devel Ther, 2017-11-23;11(0):3333-3341.
Species: Human
Sample Types: Cell Culture Supernates
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Design, synthesis, docking and QSAR study of substituted benzimidazole linked oxadiazole as cytotoxic agents, EGFR and erbB2 receptor inhibitors
Authors: Md Jawaid Akhtar
Eur J Med Chem, 2016-12-09;126(0):853-869.
Species: Human
Sample Types: Cell Culture Supernates
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The HER2-binding affibody molecule (Z(HER2ratio342))(2) increases radiosensitivity in SKBR-3 cells.
Authors: Ekerljung L, Lennartsson J, Gedda L
PLoS ONE, 2012-11-14;7(11):e49579.
Species: Human
Sample Types: Cell Lysates
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Combined vaccination with HER-2 peptide followed by therapy with VEGF peptide mimics exerts effective anti-tumor and anti-angiogenic effects in vitro and in vivo.
Authors: Foy, Kevin C, Miller, Megan J, Moldovan, Nicanor, Carson, William, Kaumaya, Pravin T
Oncoimmunology, 2012-10-01;1(7):1048-1060.
Species: Human
Sample Types: Cell Culture Supernates
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Cellular Effects of HER3-Specific Affibody Molecules.
Authors: Gostring L, Malm M, Hoiden-Guthenberg I, Frejd FY, Stahl S, Lofblom J, Gedda L
PLoS ONE, 2012-06-29;7(6):e40023.
Species: Human
Sample Types: Cell Lysates
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Site-specific coupling and sterically controlled formation of multimeric antibody fab fragments with unnatural amino acids.
Authors: Hutchins BM, Kazane SA, Staflin K, Forsyth JS, Felding-Habermann B, Schultz PG, Smider VV
J. Mol. Biol., 2011-01-13;406(4):595-603.
Species: Human
Sample Types: Cell Lysates
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AZD8931, an equipotent, reversible inhibitor of signaling by epidermal growth factor receptor, ERBB2 (HER2), and ERBB3: a unique agent for simultaneous ERBB receptor blockade in cancer.
Authors: Hickinson DM, Klinowska T, Speake G
Clin. Cancer Res., 2010-02-09;16(4):1159-69.
Species: Human
Sample Types: Cell Lysates
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Peptide vaccines of the HER-2/neu dimerization loop are effective in inhibiting mammary tumor growth in vivo.
Authors: Allen SD, Garrett JT, Rawale SV, Jones AL, Phillips G, Forni G, Morris JC, Oshima RG, Kaumaya PT
J. Immunol., 2007-07-01;179(1):472-82.
Species: Human
Sample Types: Cell Lysates
FAQs
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Does the capture antibody in the Human Phospho-ErbB2/Her2 DuoSet IC ELISA, Catalog # DYC1768, detect Erb2 or phosphorylated Erb2?
The capture antibody detects and binds both phosphorylated and unphosphorylated Erb2. The conjugated detection antibody specific for phosphorylated tyrosine will detect only the phosphorylated protein.
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Which phosphorylated sites are recognized by this assay?
This assay utilizes an anti-phosphorylated tyrosine monoclonal detection antibody, and it recognizes all phosphorylated tyrosine residues.
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Does this assay detect both phosphorylated and non-phosphorylated Erb2?
The capture antibody binds both phosphorylated and non-phosphorylated Erb2. The conjugated detection antibody is specific for phosphorylated tyrosine so the assay will detect only phosphorylated Erb2.
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