Human Phospho-p27/Kip1 (T198) Antibody

(12 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human p27/Kip1 when phosphorylated at T198. Does not recognize p27/Kip1 when unphosphorylated at T198.
  • Source
    Polyclonal Rabbit IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Phosphopeptide containing human p27/Kip1 T198 site
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.5 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human Phospho-p27/Kip1 (T198) by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p27/Kip1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27/Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4‑imidazolecarboxamide riboside) or glucose withdrawal.

  • Long Name:
    p27 Cyclin Dependent Kinase 4 Inhibitor 1B
  • Entrez Gene IDs:
    1027 (Human); 12576 (Mouse); 83571 (Rat)
  • Alternate Names:
    CDKN1B; CDKN4; cyclin-dependent kinase inhibitor 1B (p27, Kip1); cyclin-dependent kinase inhibitor 1B; Cyclin-dependent kinase inhibitor p27; Kip1; KIP1P27KIP1; MEN1B; MEN4; p27; p27Kip1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

12 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Voluntary physical activity counteracts the proliferative tumor growth microenvironment created by adipose tissue via high-fat diet feeding in female rats
    Authors: CF Theriau, MK Connor
    Physiol Rep, 2017;5(13):.
    Species: Human
    Sample Type: Cell Lystaes
    Application: WB
  2. Cytoplasmic p27 promotes epithelial-mesenchymal transition and tumor metastasis via STAT3-mediated Twist1 upregulation.
    Authors: Zhao D, Besser A, Wander S, Sun J, Zhou W, Wang B, Ince T, Durante M, Guo W, Mills G, Theodorescu D, Slingerland J
    Oncogene, 2015;34(43):5447-59.
    Species: Human
    Sample Type: Cell Lysates
    Application: IP
  3. Antitumoral activity of lenalidomide in in vitro and in vivo models of mantle cell lymphoma involves the destabilization of cyclin D1/p27KIP1 complexes.
    Authors: Moros A, Bustany S, Cahu J, Saborit-Villarroya I, Martinez A, Colomer D, Sola B, Roue G
    Clin Cancer Res, 2014;20(2):393-403.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
  4. TRIP6 regulates p27 KIP1 to promote tumorigenesis.
    Authors: Lin, Victor T, Lin, Vivian Y, Lai, Yun-Ju, Chen, Chen-Sha, Liu, Kang, Lin, Weei-Chi, Lin, Fang-Tsy
    Mol Cell Biol, 2013;33(7):1394-409.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. Cdc6 protein activates p27KIP1-bound Cdk2 protein only after the bound p27 protein undergoes C-terminal phosphorylation.
    Authors: Uranbileg B, Yamamoto H, Park JH
    J. Biol. Chem., 2012;287(9):6275-83.
    Species: Rat
    Sample Type: Cell Lysates
    Application: WB
  6. A novel p38-MAPK signaling axis modulates neutrophil biology in head and neck cancer.
    Authors: Dumitru CA, Fechner MK, Hoffmann TK
    J. Leukoc. Biol., 2012;91(4):591-8.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. Promotion of cytoplasmic mislocalization of p27 by Helicobacter pylori in gastric cancer.
    Authors: Wen S, So Y, Singh K
    Oncogene, 2012;31(14):1771-80.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. The microbiome and butyrate regulate energy metabolism and autophagy in the mammalian colon.
    Authors: Donohoe DR, Garge N, Zhang X, Sun W, O'Connell TM, Bunger MK, Bultman SJ
    Cell Metab., 2011;13(5):517-26.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  9. Cyclin D1 regulates p27(Kip1) stability in B cells.
    Authors: Bustany S, Tchakarska G, Sola B
    Cell. Signal., 2011;23(1):171-9.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  10. Ablation of Akt2 induces autophagy through cell cycle arrest, the downregulation of p70S6K, and the deregulation of mitochondria in MDA-MB231 cells.
    Authors: Santi SA, Lee H
    PLoS ONE, 2011;6(1):e14614.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  11. Homeodomain-interacting Protein Kinase-2 Stabilizes p27kip1 by Its Phosphorylation at Serine 10 and Contributes to Cell Motility.
    Authors: Pierantoni GM, Esposito F, Tornincasa M, Rinaldo C, Viglietto G, Soddu S, Fusco A
    J. Biol. Chem., 2011;286(33):29005-13.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  12. Cytoplasmic sequestration of p27 via AKT phosphorylation in renal cell carcinoma.
    Authors: Kim J, Jonasch E, Alexander A, Short JD, Cai S, Wen S, Tsavachidou D, Tamboli P, Czerniak BA, Do KA, Wu KJ, Marlow LA, Wood CG, Copland JA, Walker CL
    Clin. Cancer Res., 2009;15(1):81-90.
    Species: Human
    Sample Type: Tissue Homogenates
    Application: WB
Expand to show all 12 Citations
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