Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Rat

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all p27/Kip1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human p27/Kip1 T198 site

Specificity

Detects human p27/Kip1 when phosphorylated at T198. Does not recognize p27/Kip1 when unphosphorylated at T198.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-p27/Kip1 (T198) Antibody

Detection of Human Phospho-p27/Kip1 (T198) antibody by Western Blot.

Detection of Human Phospho-p27/Kip1 (T198) by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, non-transfected, transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A). PVDF membrane was probed with 0.5 µg/mL Rabbit Anti-Human Phospho-p27/Kip1 (T198) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3994) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for p27/Kip1 was detected at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Phospho-p27/Kip1 (T198) Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: MCF‑7 human breast cancer cell line transfected with plasmid expressing YFP-p27/Kip1 wild type (WT), or transfected with YFP-p27/Kip1 T198A mutant (T198A)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: p27/Kip1

The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle. p27/Kip1 is phosphorylated at T198 in cells treated with the AMP-dependent protein kinase (AMPK) activator AICAR (5-amino-4‑imidazolecarboxamide riboside) or glucose withdrawal.

Long Name

p27 Cyclin Dependent Kinase 4 Inhibitor 1B

Alternate Names

CDKN1B, Kip1

Entrez Gene IDs

1027 (Human); 12576 (Mouse); 83571 (Rat)

Gene Symbol

CDKN1B

Additional p27/Kip1 Products

Product Documents for Human Phospho-p27/Kip1 (T198) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Phospho-p27/Kip1 (T198) Antibody

For research use only

Related Research Areas

Autophagy
Cancer Biomarkers
Cell Cycle

Citations for Human Phospho-p27/Kip1 (T198) Antibody

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