Human Phospho-p38 alpha (T180/Y182) Antibody

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Human Phospho-p38 alpha  (T180/Y182) Antibody in Data
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Human Phospho-p38 alpha (T180/Y182) Antibody Summary

Species Reactivity
Detects human p38 alpha when phosphorylated at T180/Y182.
Monoclonal Rat IgG2B Clone # 948243
Protein A or G purified from hybridoma culture supernatant
Phosphopeptide containing human p38 alpha T180/Y182 site
Accession # Q16539
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.


Recommended Concentration
5-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Phospho-p38 alpha (T180/Y182) in HeLa Human Cell Line. View Larger

Phospho-p38 alpha (T180/Y182) in HeLa Human Cell Line. p38a phosphorylated at T180/Y182 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line, untreated or treated with calf intestinal phosphatase, using Rat Anti-Human Phospho-p38a (T180/Y182) Monoclonal Antibody (Catalog # MAB8692) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p38 alpha

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses, such as UV irradiation and heat shock. Stress signals are delivered to this cascade by members of small GTPases of the Rho family (Rac, Rho, Cdc42). p38 MAPK is involved in the regulation of Hsp27 and MAPKAP-2 and several transcription factors including ATF2, STAT1, and indirectly CREB via activation of MSK1. The p38 MAPK protein also plays a role in cell differentiation, autophagy and apoptosis. Mkk3 and SEK can activate p38 MAPK by phosphorylation at Thr180 and Tyr182, which in turn activates the MAPKAP kinase 2 and regulating phosphorylation of ATF2, Mac and MEF2.

Entrez Gene IDs
1432 (Human); 26416 (Mouse); 81649 (Rat)
Alternate Names
MAPK14; p38 alpha; SAPK2a

Product Datasheets


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Cell and Tissue Staining Kits

Isotype Controls

Rat IgG2B Isotype Control

Reconstitution Buffers

Secondary Antibodies

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