Detection of Human Phospho-Rad17 (S635) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 25 μM Etoposide for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Rad17 (S635) Polyclonal Antibody (Catalog # MAB1374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Rad17 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. The Rad genes were first identified in yeast as genes required for the DNA damage response. Human Rad17 bears homology to the replication factor C (RFC) proteins and interacts with the Rad9/Rad1/Hus1 complex in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).
Entrez Gene IDs:
5884 (Human); 310034 (Rat)
CCYC; cell cycle checkpoint protein (RAD17); cell cycle checkpoint protein RAD17; FLJ41520; HRAD17; R24L; RAD1 (S. pombe) homolog; RAD1 homolog; RAD17 homolog (S. pombe); Rad17; Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog
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