Human Phospho-Rad17 (S635) Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB1374
MAB1374-SP
Detection  of Human Phospho-Rad17 (S635) by Western Blot.
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Human Phospho-Rad17 (S635) Antibody Summary

Species Reactivity
Human
Specificity
Detects human Rad17 when phosphorylated at S635 in Western blots.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1244A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Phosphopeptide containing the human Rad17 (S635)
Accession # O75943
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.5 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection  of Human Phospho-Rad17 (S635) by Western Blot. View Larger

Detection of Human Phospho-Rad17 (S635) by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 25 μM Etoposide for 6 hours. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-Rad17 (S635) Polyclonal Antibody (Catalog # MAB1374) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Rad17 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rad17

The DNA damage response activates cell cycle checkpoints to allow time for DNA repair and ensure the fidelity of each cell cycle. The Rad genes were first identified in yeast as genes required for the DNA damage response. Human Rad17 bears homology to the replication factor C (RFC) proteins and interacts with the Rad9/Rad1/Hus1 complex in cells exposed to multiple types of genotoxic stress, including ionizing radiation (IR) and ultraviolet light (UV).

Entrez Gene IDs
5884 (Human); 310034 (Rat)
Alternate Names
CCYC; cell cycle checkpoint protein (RAD17); cell cycle checkpoint protein RAD17; FLJ41520; HRAD17; R24L; RAD1 (S. pombe) homolog; RAD1 homolog; RAD17 homolog (S. pombe); Rad17; Rad17-like protein; RAD17SP; RAD24; RF-C activator 1 homolog; RF-C/activator 1 homolog

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