Human Phospho-STAT2 (Y690) Antibody

R&D Systems | Catalog # MAB2890

Recombinant Monoclonal Antibody.
R&D Systems
100% Guarantee

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1021D
View all STAT2 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing the human STAT2 Y690 site

Specificity

Detects human phospho-STAT2 (Y690) in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human Phospho-STAT2 (Y690) Antibody

Detection of Human Phospho-STAT2 (Y689) antibody by Western Blot.

Detection of Human Phospho-STAT2 (Y690) by Western Blot.

Western blot shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes. PVDF membrane was probed with 0.05 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for Phospho-STAT2 (Y690) at approximately 113 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-STAT2 (Y689) antibody by Simple WesternTM.

Detection of Human Phospho-STAT2 (Y690) by Simple WesternTM.

Simple Western lane view shows lysates of Daudi human Burkitt's lymphoma cell line untreated (-) or treated (+) with 500 U/mL Recombinant Human IFN-aA (11100-1) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for Phospho-STAT2 (Y690) at approximately 111 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human Phospho-STAT2 (Y690) Monoclonal Antibody (Catalog # MAB2890). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Human STAT2 by Western Blot

Detection of Human STAT2 by Western Blot

Induction of type I IFN signaling by viral and nonviral stimuli is inhibited in G2/M-arrested cells. Suit2 cells were treated for 25 h with the vehicle, paclitaxel, colchicine, or ruxolitinib at 500 nM. Cells were then treated with the vehicle (untreated), TransIT reagent (0.5%, vol/vol), poly(I:C) at 10 μg/ml plus TransIT reagent, IFN-alpha at 5,000 U/ml, or VSV at an MOI of 30 based on titration on BHK-21 cells. VSV was aspirated 1 h later, and medium was added to infected wells. Cells remained in treatment for a total of 4 h, after which total protein was isolated. Western blot results for STAT1 and -2 proteins and their phosphorylated forms are shown in addition to VSV proteins. GAPDH was used to confirm that protein loading was the same across the gel. Protein names and protein sizes in kilodaltons are indicated on the left and right, respectively. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30487274), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Phospho-STAT2 (Y690) Antibody

Application
Recommended Usage

Simple Western

0.5 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alpha A (Catalog # 11100-1)

Western Blot

0.05 µg/mL
Sample: Daudi human Burkitt's lymphoma cell line treated with Recombinant Human IFN‑ alpha A (Catalog # 11100-1)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: STAT2

STAT2 (signal transducer and activator of transcription 2) is a 113 kDa member of the STAT family of cytoplasmic transcription factors. STAT members generally mediate cytokine, growth factor and hormone receptor signal transduction. STAT2 is associated with type I ( alpha - and beta -) interferon signaling. All STATs contain an N‑terminal oligomerization domain, a DNA-binding domain, and an SH2-association region. STAT2 is phosphorylated at Y690 by receptor-associated Janus kinases (JAKs) leading to STAT2 dimerization and subsequent translocation to the nucleus to activate gene transcription.

Long Name

Signal Transducer and Activator of Transcription 2

Alternate Names

interferon alpha induced transcriptional activator, ISGF-3, MGC59816, P113, signal transducer and activator of transcription 2, signal transducer and activator of transcription 2, 113kD, signal transducer and activator of transcription 2, 113kDa, STAT113

Entrez Gene IDs

6773 (Human); 20847 (Mouse)

Gene Symbol

STAT2

Additional STAT2 Products

Product Documents for Human Phospho-STAT2 (Y690) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human Phospho-STAT2 (Y690) Antibody

For research use only

Citations for Human Phospho-STAT2 (Y690) Antibody

Customer Reviews for Human Phospho-STAT2 (Y690) Antibody

There are currently no reviews for this product. Be the first to review Human Phospho-STAT2 (Y690) Antibody and earn rewards!

Have you used Human Phospho-STAT2 (Y690) Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs for Human Phospho-STAT2 (Y690) Antibody

Showing  1 - 1 of 1 FAQ Showing All

  • Q: Does your phospho-STAT2 antibody (clone number 1021D) cross-react with mouse protein?

    A: I have done a blast search between human and mouse STAT2, and it shows that although the tyrosine (Y) residues are conserved in the both species, the adjacent a.a. are different.  Therefore the peptide that was used to raise this antibody should contain the diverse a.a residues. Hence, I anticipate that this Ab will not cross react with mouse sample. 

Showing  1 - 1 of 1 FAQ Showing All
View all FAQs for Antibodies

Associated Pathways

Jak/STAT Signaling Pathway Jak/STAT Signaling Pathway Thumbnail