Human PI 3-Kinase p85 beta Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB6777R Datasheet / COA / SDS

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FAB6777R-100UG

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Human PI 3-Kinase p85 beta Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha, rhPI 3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.

Source

Monoclonal Mouse IgG2b Clone # 572001

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human PI 3â€‘Kinase p85Î²
Lys516-Gly642
Accession # O00459

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PI 3-Kinase p85 beta

Phosphoinositide 3-kinase p85 beta subunit (PI 3-Kinase p85 beta ), also called regulatory subunit 2 (PIK3-R2) is an 85 kDa, 728 amino acid (aa) regulatory subunit of the PI3K complex. It shares ubiquitous expression and overlapping function with the p85 alpha subunit, but the two are differentially phosphorylated and may have different effects on apoptosis. Within aa 516-642, human p85 beta shares 97%, 96% and 68% aa identity with mouse and rat p85 beta and human p85 alpha, respectively. Full-length human p85 alpha and p85 beta share 59% aa identity.

Long Name

Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta

Entrez Gene IDs

5296 (Human); 18709 (Mouse); 29741 (Rat)

Alternate Names

P85B; p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta); PI 3Kinase p85 beta; PI 3-Kinase p85 beta; PI3K regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PIK3R2; ptdIns-3-kinase regulatory subunit beta; ptdIns-3-kinase regulatory subunit p85-beta