Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha, rhPI
3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.
Monoclonal Mouse IgG2B Clone # 572001
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human PI 3‑Kinase p85 beta Lys516-Gly642 Accession # O00459
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human PI 3-Kinase p85 beta by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85 beta at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PI 3‑Kinase p85 beta in K562 Human Cell Line. PI 3‑Kinase p85 beta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human PI 3‑Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p85 beta
Phosphoinositide 3-kinase p85 beta subunit (PI 3-Kinase p85 beta ), also called regulatory subunit 2 (PIK3-R2) is an 85 kDa, 728 amino acid (aa) regulatory subunit of the PI3K complex. It shares ubiquitous expression and overlapping function with the p85 alpha subunit, but the two are differentially phosphorylated and may have different effects on apoptosis. Within aa 516-642, human p85 beta shares 97%, 96% and 68% aa identity with mouse and rat p85 beta and human p85 alpha, respectively. Full-length human p85 alpha and p85 beta share 59% aa identity.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.