Human PI 3‑Kinase p85 beta Antibody

Name: Human PI 3‑Kinase p85 beta Antibody
Brand: R&D Systems
SKU: MAB6777
Price: 449 USD
Availability: InStock

R&D Systems | Catalog # MAB6777

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # 572001

View all PI 3-Kinase p85 beta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PI 3‑Kinase p85 beta
Lys516-Gly642
Accession # O00459

Specificity

Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha, rhPI 3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human PI 3‑Kinase p85 beta Antibody

Detection of Human PI 3-Kinase p85 beta by Western Blot.

Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85 beta at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PI 3‑Kinase p85 beta in K562 Human Cell Line.

PI 3-Kinase p85 beta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human PI 3‑Kinase p85 beta Antibody

Application

Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed K562 human chronic myelogenous leukemia cell line

Western Blot

2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PI 3-Kinase p85 beta

Phosphoinositide 3-kinase p85 beta subunit (PI 3-Kinase p85 beta ), also called regulatory subunit 2 (PIK3-R2) is an 85 kDa, 728 amino acid (aa) regulatory subunit of the PI3K complex. It shares ubiquitous expression and overlapping function with the p85 alpha subunit, but the two are differentially phosphorylated and may have different effects on apoptosis. Within aa 516-642, human p85 beta shares 97%, 96% and 68% aa identity with mouse and rat p85 beta and human p85 alpha, respectively. Full-length human p85 alpha and p85 beta share 59% aa identity.