Human PI 3-Kinase p85 beta Antibody

(1 citations)   
  • Species Reactivity
    Human
  • Specificity
    Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha, rhPI 3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.
  • Source
    Monoclonal Mouse IgG2B Clone # 572001
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human PI 3‑Kinase p85 beta
    Lys516-Gly642
    Accession # O00459
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PI 3-Kinase p85 beta by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85 beta at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
PI 3‑Kinase p85 beta in K562 Human Cell Line. PI 3‑Kinase p85 beta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human PI 3‑Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p85 beta
Phosphoinositide 3-kinase p85 beta subunit (PI 3-Kinase p85 beta ), also called regulatory subunit 2 (PIK3-R2) is an 85 kDa, 728 amino acid (aa) regulatory subunit of the PI3K complex. It shares ubiquitous expression and overlapping function with the p85 alpha subunit, but the two are differentially phosphorylated and may have different effects on apoptosis. Within aa 516-642, human p85 beta shares 97%, 96% and 68% aa identity with mouse and rat p85 beta and human p85 alpha, respectively. Full-length human p85 alpha and p85 beta share 59% aa identity.
  • Long Name:
    Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta
  • Entrez Gene IDs:
    5296 (Human); 18709 (Mouse); 29741 (Rat)
  • Alternate Names:
    P85B; p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta); PI 3Kinase p85 beta; PI 3-Kinase p85 beta; PI3K regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PIK3R2; ptdIns-3-kinase regulatory subunit beta; ptdIns-3-kinase regulatory subunit p85-beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. A large scale Huntingtin protein interaction network implicates Rho GTPase signaling pathways in Huntington disease.
    Authors: Tourette, Cendrine, Li, Biao, Bell, Russell, O'Hare, Shannon, Kaltenbach, Linda S, Mooney, Sean D, Hughes, Robert E
    J Biol Chem, 0;289(10):6709-26.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
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