Human PI 3-Kinase p85 beta Antibody

Catalog # Availability Size / Price Qty
MAB6777
MAB6777-SP
PI 3‑Kinase p85 beta  in K562 Human Cell Line.
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Product Details
Citations (1)
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Human PI 3-Kinase p85 beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human PI 3‑Kinase p85 beta in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) PI 3-Kinase p85 beta (aa 325-428), rhPI 3-Kinase p85 alpha, rhPI 3-Kinase p87, rhPIK3R4, or rhPIK3R5 is observed.
Source
Monoclonal Mouse IgG2B Clone # 572001
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human PI 3‑Kinase p85 beta
Lys516-Gly642
Accession # O00459
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunocytochemistry PI 3-Kinase p85 beta  antibody in K562 Human Cell Line by Immunocytochemistry (ICC). View Larger

PI 3‑Kinase p85 beta in K562 Human Cell Line. PI 3-Kinase p85 beta was detected in immersion fixed K562 human chronic myelogenous leukemia cell line using Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Western Blot Detection of Human PI 3-Kinase p85 beta  antibody by Western Blot. View Larger

Detection of Human PI 3-Kinase p85 beta by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human PI 3-Kinase p85 beta Monoclonal Antibody (Catalog # MAB6777) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PI 3-Kinase p85 beta at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PI 3-Kinase p85 beta

Phosphoinositide 3-kinase p85 beta subunit (PI 3-Kinase p85 beta ), also called regulatory subunit 2 (PIK3-R2) is an 85 kDa, 728 amino acid (aa) regulatory subunit of the PI3K complex. It shares ubiquitous expression and overlapping function with the p85 alpha subunit, but the two are differentially phosphorylated and may have different effects on apoptosis. Within aa 516-642, human p85 beta shares 97%, 96% and 68% aa identity with mouse and rat p85 beta and human p85 alpha, respectively. Full-length human p85 alpha and p85 beta share 59% aa identity.

Long Name
Phosphatidylinositol-3-kinase 85 kDa Regulatory Subunit beta
Entrez Gene IDs
5296 (Human); 18709 (Mouse); 29741 (Rat)
Alternate Names
P85B; p85-beta; phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta; phosphatidylinositol 3-kinase regulatory subunit beta; phosphatidylinositol 3-kinase, regulatory subunit, polypeptide 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit 2 (beta); phosphoinositide-3-kinase, regulatory subunit 2 (p85 beta); phosphoinositide-3-kinase, regulatory subunit, polypeptide 2 (p85 beta); PI 3Kinase p85 beta; PI 3-Kinase p85 beta; PI3K regulatory subunit beta; PI3-kinase regulatory subunit beta; PI3-kinase subunit p85-beta; PIK3R2; ptdIns-3-kinase regulatory subunit beta; ptdIns-3-kinase regulatory subunit p85-beta

Product Datasheets

Citation for Human PI 3-Kinase p85 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. A large scale Huntingtin protein interaction network implicates Rho GTPase signaling pathways in Huntington disease.
    Authors: Tourette, Cendrine, Li, Biao, Bell, Russell, O'Hare, Shannon, Kaltenbach, Linda S, Mooney, Sean D, Hughes, Robert E
    J Biol Chem, 0;289(10):6709-26.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Isotype Controls

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Secondary Antibodies

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