Human PLK3 Antibody

Catalog # Availability Size / Price Qty
AF4197
AF4197-SP
Detection of Human PLK3 by Western Blot.
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Human PLK3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PLK3 in Western blots.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human PLK3
Pro320-Ala520
Accession # Q9H4B4.2
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human PLK3 by Western Blot. View Larger

Detection of Human PLK3 by Western Blot. Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PLK3 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLK3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PLK3

Polo-like kinases are critical regulators of cell cycle progression, cytokinesis, mitosis, and the genotoxic stress response. Four distinct members have been identified to date in mammalian cells: PLK1, PLK2, PLK3, and PLK4. PLK3 (polo-like kinase 3) plays an important role in cell cycle regulation by modulating microtubule dynamics and centrosomal function. Although protein levels are unchanged throughout the cell cycle, kinase activity peaks during late S and G2 phases. Many key cell cycle regulators are phosphorylated by PLK3 including p53 on Ser20, Cdc25C on Ser216, and the major subunit of DNA polymerase δ, p125 on Ser60.

Long Name
Polo-like Kinase 3
Entrez Gene IDs
1263 (Human); 12795 (Mouse); 58936 (Rat)
Alternate Names
CNK; CNKpolo-like kinase 3 (Drosophila); cytokine-inducible kinase; Cytokine-inducible serine/threonine-protein kinase; EC 2.7.11; FNK; FNKEC 2.7.11.21; PLK3; polo-like kinase 3PLK-3; PRK; PRKFGF-inducible kinase; Proliferation-related kinase; serine/threonine-protein kinase PLK3

Product Datasheets

FAQs

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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