Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PLK3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PLK3
Pro320-Ala520
Accession # Q9H4B4.2

Specificity

Detects human PLK3 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PLK3 Antibody

Detection of Human PLK3 antibody by Western Blot.

Detection of Human PLK3 by Western Blot.

Western blot shows nuclear extracts of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PLK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PLK3 at approximately 72 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human PLK3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PLK3

Polo-like kinases are critical regulators of cell cycle progression, cytokinesis, mitosis, and the genotoxic stress response. Four distinct members have been identified to date in mammalian cells: PLK1, PLK2, PLK3, and PLK4. PLK3 (polo-like kinase 3) plays an important role in cell cycle regulation by modulating microtubule dynamics and centrosomal function. Although protein levels are unchanged throughout the cell cycle, kinase activity peaks during late S and G2 phases. Many key cell cycle regulators are phosphorylated by PLK3 including p53 on Ser20, Cdc25C on Ser216, and the major subunit of DNA polymerase delta, p125 on Ser60.

Long Name

Polo-like Kinase 3

Alternate Names

CNK, FNK, PRK

Entrez Gene IDs

1263 (Human); 12795 (Mouse); 58936 (Rat)

Gene Symbol

PLK3

UniProt

Q9H4B4.2

Additional PLK3 Products

Product Documents for Human PLK3 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PLK3 Antibody

For research use only

Related Research Areas

Intracellular Kinases

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