Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all PP2C gamma Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PP2C gamma
Met1-Asp546
Accession # O15355

Specificity

Detects endogenous human PP2C gamma in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human PP2C gamma Antibody

Detection of Human PP2C? antibody by Western Blot.

Detection of Human PP2C gamma by Western Blot.

Western blot shows lysates of HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PP2C gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5595) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP2C gamma at approximately 72 to 75 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.

Applications for Human PP2C gamma Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PP2C gamma

PP2C gamma (Protein phosphatase 2C gamma; also PPM1G) is a nuclear member of the metal ion-dependent PP2C family of Ser/Thr protein phosphatases. Although its predicted MW is 59 kDa, it runs anomalously at 72‑75 kDa in SDS-PAGE. PP2C gamma is widely expressed and serves as a negative regulator of the cell stress response. In particular, it dephosphorylates histones H2A and H2B, allowing for their placement into chromatin. This is important when histones are reincorporated into DNA following DNA repair. Human PP2C gamma is 546 amino acids (aa) in length. It contains a catalytic domain (aa 27‑120), an Asp/Glu-rich region (aa 258‑326) and a poly-Lys tail (aa 539‑544). One potential splice form shows a Phe substitution for aa 20‑38.

Long Name

Protein Phosphatase 2C gamma

Alternate Names

PP2CG, PPM1C, PPM1G, PPP2CG

Entrez Gene IDs

5496 (Human); 14208 (Mouse); 259229 (Rat)

Gene Symbol

PPM1G

UniProt

O15355 (Human)

Additional PP2C gamma Products

Product Documents for Human PP2C gamma Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PP2C gamma Antibody

For research use only

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