Human PP2C gamma Antibody

    
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  • Species Reactivity
    Human
  • Specificity
    Detects endogenous human PP2C gamma in Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PP2C gamma
    Met1-Asp546
    Accession # O15355
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of Human PP2C gamma by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells, MCF-7 human breast cancer cell line, and Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Human PP2C gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5595) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PP2C gamma at approximately 72 to 75 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PP2C gamma

PP2C gamma (Protein phosphatase 2C gamma; also PPM1G) is a nuclear member of the metal ion-dependent PP2C family of Ser/Thr protein phosphatases. Although its predicted MW is 59 kDa, it runs anomalously at 72‑75 kDa in SDS-PAGE. PP2C gamma is widely expressed and serves as a negative regulator of the cell stress response. In particular, it dephosphorylates histones H2A and H2B, allowing for their placement into chromatin. This is important when histones are reincorporated into DNA following DNA repair. Human PP2C gamma is 546 amino acids (aa) in length. It contains a catalytic domain (aa 27‑120), an Asp/Glu-rich region (aa 258‑326) and a poly-Lys tail (aa 539‑544). One potential splice form shows a Phe substitution for aa 20‑38.

  • Long Name:
    Protein Phosphatase 2C gamma
  • Entrez Gene IDs:
    5496 (Human); 14208 (Mouse); 259229 (Rat)
  • Alternate Names:
    EC 3.1.3.16; MGC1675; MGC2870; PP2C gamma; PP2C, gamma; PP2CG; PP2CGAMMA; PP2C-gamma; PPM1C; PPM1G; PPP2CG; Protein phosphatase 1C; protein phosphatase 1G (formerly 2C), magnesium-dependent, gamma isoform; protein phosphatase 1G; protein phosphatase 2, catalytic subunit, gamma isoform; protein phosphatase 2C gamma isoform; Protein phosphatase 2C isoform gamma; Protein phosphatase magnesium-dependent 1 gamma; protein phosphatase, Mg2+/Mn2+ dependent, 1G
Related Research Areas

FAQs

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Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 204  
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Reconstitution Buffers
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Reconstitution Buffer 1 (PBS)

RB01
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB , Simple Western HAF109 20  
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Goat IgG HRP-conjugated Antibody

WB HAF017 16  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow , IHC , ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow , IHC , ICC NL003 6
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 7  
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow , IHC , ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB , ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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