Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2297B
View all PPAR alpha/NR1C1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant  human PPAR alpha /NR1C1
Met1-Asn99
Accession # Q07869

Specificity

Detects human PPAR alpha /NR1C1 in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human PPAR alpha /NR1C1 Antibody

PPARa/NR1C1 antibody in HT-29 Human Cell Line by Immunocytochemistry (ICC).

PPAR alpha /NR1C1 in HT-29 Human Cell Line.

PPARa/NR1C1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rabbit Anti-Human PPARa/NR1C1 Monoclonal Antibody (Catalog # MAB24781) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human PPAR alpha /NR1C1 Antibody

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed HT-29 human colon adenocarcinoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PPAR alpha/NR1C1

Peroxisome proliferator activated receptor alpha (PPARA) a member of the steroid hormone receptor superfamily, is a nuclear transcription factor which mediates the activity of peroxisome proliferators. PPAR alpha exhibits the highest affinity with unsaturated fatty acids, and linolenic acids. PPAR alpha is expressed in brown fat, liver, kidney, heart, mucosa of the stomach and duodenum, retina, adrenal gland, skeletal muscle, pancreatic islets and smooth muscle cells. PPAR alpha plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease.

Long Name

Peroxisome Proliferator-activated Receptor alpha

Alternate Names

NR1C1, PPARA

Entrez Gene IDs

5465 (Human)

Gene Symbol

PPARA

UniProt

Q07869

Additional PPAR alpha/NR1C1 Products

Product Documents for Human PPAR alpha /NR1C1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PPAR alpha /NR1C1 Antibody

For research use only

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