Human PPAR alpha /NR1C1 Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB24781-100
MAB24781-SP
Human PPAR alpha /NR1C1 Antibody in Immunocytochemistry
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Human PPAR alpha /NR1C1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human PPAR alpha /NR1C1 in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 2297B
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant  human PPAR alpha /NR1C1
Met1-Asn99
Accession # Q07869
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
3-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry PPAR alpha /NR1C1 in HT-29 Human Cell Line. View Larger

PPAR alpha /NR1C1 in HT-29 Human Cell Line. PPARa/NR1C1 was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Rabbit Anti-Human PPARa/NR1C1 Monoclonal Antibody (Catalog # MAB24781) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PPAR alpha/NR1C1

Peroxisome proliferator activated receptor alpha (PPARA) a member of the steroid hormone receptor superfamily, is a nuclear transcription factor which mediates the activity of peroxisome proliferators. PPAR alpha exhibits the highest affinity with unsaturated fatty acids, and linolenic acids. PPAR alpha is expressed in brown fat, liver, kidney, heart, mucosa of the stomach and duodenum, retina, adrenal gland, skeletal muscle, pancreatic islets and smooth muscle cells. PPAR alpha plays important roles in lipid and glucose metabolism, and has been implicated in obesity-related metabolic diseases such as hyperlipidemia, insulin resistance, and coronary artery disease.

Long Name
Peroxisome Proliferator-activated Receptor alpha
Entrez Gene IDs
5465 (Human)
Alternate Names
alpha; hPPAR; NR1C1; NR1C1MGC2237; Nuclear receptor subfamily 1 group C member 1; peroxisome proliferator-activated receptor alpha; PPAR alpha; PPARA; PPARalpha; PPAR-alpha; PPARMGC2452

Product Datasheets

FAQs

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Cell and Tissue Staining Kits

Isotype Controls

Normal Rabbit IgG Control

Ctrl,CyTOF-ready

Reconstitution Buffers

Secondary Antibodies

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