Human/Primate BMP-4 Biotinylated Antibody

Catalog #: BAM7572 Datasheet / COA / SDS

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BAM7572

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Human/Primate BMP-4 Biotinylated Antibody Summary

Species Reactivity

Human, Primate

Specificity

Detects human and primate BMP-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human BMP‑2, ‑3, ‑5, ‑6, or ‑7.

Source

Monoclonal Mouse IgG₁ Clone # 66108

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human BMP‑4
Ser293-Arg408
Accession # P12644

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human BMP‑4 (Catalog # 314-BP)

Human/Primate BMP-4 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.5-2.0 µg/mL

Use in combination with:

Capture Reagent: Human/Primate BMP‑4 Antibody (Catalog # MAB7571)

Standard: Recombinant Human BMP-4 Protein (Catalog # 314-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMP-4 is one of at least 20 structurally and functionally related BMPs which are members of the TGF-beta superfamily. Biologically active BMP-4 is a disulfide-linked homodimer of the carboxy-terminal domain. The morphogenetic effects of BMP-4 on various tissues are mediated by type I and type II BMP receptors.

Long Name

Bone Morphogenetic Protein 4

Entrez Gene IDs

652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)

Alternate Names

BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME