Human/Primate BMP-4 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAM7572

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Human/Primate BMP-4 Biotinylated Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate BMP-4 in direct ELISAs and Western blots. Does not cross-react with recombinant human BMP‑2, ‑3, ‑5, ‑6, or ‑7.
Source
Monoclonal Mouse IgG1 Clone # 66108
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMP‑4
Ser293-Arg408
Accession # P12644
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BMP‑4 (Catalog # 314-BP)

Human/Primate BMP-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.5-2.0 µg/mL 

Use in combination with:

Capture Reagent: Human/Primate BMP‑4 Antibody (Catalog # MAB7571)

Standard: Recombinant Human BMP-4 Protein (Catalog # 314-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMP-4 is one of at least 20 structurally and functionally related BMPs which are members of the TGF-beta  superfamily. Biologically active BMP-4 is a disulfide-linked homodimer of the carboxy-terminal domain. The morphogenetic effects of BMP-4 on various tissues are mediated by type I and type II BMP receptors.

Long Name
Bone Morphogenetic Protein 4
Entrez Gene IDs
652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names
BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME

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