Human/Primate BMP-4 Antibody

Catalog # Availability Size / Price Qty
MAB7571-SP
MAB7571-100
MAB7571-500
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews

Human/Primate BMP-4 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate BMP-4 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) BMP‑2, rhBMP‑3, rhBMP‑5, rhBMP‑6, or rhBMP‑7 under non-reducing conditions.
Source
Monoclonal Mouse IgG2B Clone # 66110
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BMP‑4
Ser293-Arg408
Accession # P12644
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human BMP‑4 (Catalog # 314-BP) under non-reducing conditions only

Human/Primate BMP-4 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate BMP‑4 Biotinylated Antibody (Catalog # BAM7572)

Standard: Recombinant Human BMP-4 Protein (Catalog # 314-BP)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-4

BMPs are secreted signaling molecules that comprise a subfamily of the TGF-beta superfamily and were originally identified as regulators of cartilage and bone formation. There are at least 20 structurally and functionally related BMPs, most of which play roles in embryogenesis and morphogenesis of various tissues and organs. Biologically active BMPs are usually homodimers containing a characteristic cysteine knot structure. Heterodimers, BMP-2/BMP-7 and BMP-4/BMP-7 have also been suggested to exist and function in vivo. They are more potent inducers of bone formation than their respective homodimers. In addition, heterodimers, but not homodimers, are ventral mesoderm inducers. Heterodimer activity may be mediated by a different or additional receptor subtype.

Decapentaplegic (Dpp) is one of at least five TGF-beta superfamily ligands identified in the Drosophila genome. Dpp, a functional ortholog of mammalian BMP-2 and BMP-4, is a morphogen and plays an essential role in Drosophila development. Dpp regulates embryonic dorsal-ventral polarity and is required for gut morphogenesis and outgrowth and patterning of imaginal disks.

Long Name
Bone Morphogenetic Protein 4
Entrez Gene IDs
652 (Human); 12159 (Mouse); 25296 (Rat); 30612 (Zebrafish)
Alternate Names
BMP2B; BMP-2B; BMP2B1; BMP2BMCOPS6; BMP4; BMP-4; Bone morphogenetic protein 2B; bone morphogenetic protein 4; DVR4; MCOPS6; OFC11; ZYME

Product Datasheets

Citations for Human/Primate BMP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Crossveinless 2 regulates bone morphogenetic protein 9 in human and mouse vascular endothelium.
    Authors: Yao Y, Jumabay M, Ly A, Radparvar M, Wang AH, Abdmaulen R, Bostrom KI
    Blood, 2012;119(21):5037-47.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Immunoprecipitation
  2. Regulation of retinal progenitor cell differentiation by bone morphogenetic protein 4 is mediated by the smad/id cascade.
    Authors: Du Y, Xiao Q, Yip HK
    Invest. Ophthalmol. Vis. Sci., 2010;51(7):3764-73.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. Targeting of bone morphogenetic protein growth factor complexes to fibrillin.
    Authors: Sengle G, Charbonneau NL, Ono RN, Sasaki T, Alvarez J, Keene DR, Bachinger HP, Sakai LY
    J. Biol. Chem., 2008;283(20):13874-88.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Western Blot
  4. Bone morphogenetic protein-6 promotes osteoblastic prostate cancer bone metastases through a dual mechanism.
    Authors: Dai J, Keller J, Zhang J, Lu Y, Yao Z, Keller ET
    Cancer Res., 2005;65(18):8274-85.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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Reconstitution Buffers

Secondary Antibodies

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