Human/Primate IL-6 Antibody

Catalog # Availability Size / Price Qty
MAB206-SP
MAB206-100
MAB206-500
    Detection  of Recombinant Human IL‑6 by Western Blot.
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Product Details
Citations (57)
FAQs
Supplemental Products
Reviews (10)

Human/Primate IL-6 Antibody Summary

Species Reactivity
Human, Primate
Specificity
Detects human and primate IL-6 in ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse (rm) IL‑6, rhOSM, rhLIF, rhIL‑11, rhgp130, or rhCNTF.
Source
Monoclonal Mouse IgG1 Clone # 6708
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human IL-6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Immunohistochemistry
8-25 µg/mL
See below

Human/Primate IL-6 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Human/Primate IL‑6 Biotinylated Antibody (Catalog # BAF206)

Standard: Recombinant Human IL-6 Protein (Catalog # 206-IL)

Neutralization
Measured by its ability to neutralize IL‑6-induced proliferation in the T1165.85.2.1 mouse plasmacytoma cell line. Nordan, R.P. and M. Potter (1986) Science 233:566. The Neutralization Dose (ND50) is typically 0.05-0.15 µg/mL in the presence of 2.5 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection  of Recombinant Human IL‑6 by Western Blot. View Larger

Detection of Recombinant Human IL‑6 by Western Blot. Western blot shows 25 ng of Recombinant Human IL‑6 (Catalog #
206-IL), Recombinant Mouse IL‑6 (Catalog # 406-ML) and Recombinant Rat IL‑6 (Catalog # 506-RL). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/
Primate IL‑6 Monoclonal Antibody (Catalog # MAB206) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL‑6 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry IL‑6  in Human Skin. View Larger

IL‑6 in Human Skin. IL‑6 was detected in immersion fixed frozen sections of hyperplastic human skin using Mouse Anti-Human/Primate IL‑6 Monoclonal Antibody (Catalog # MAB206) at 8 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Neutralization Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. View Larger

Cell Proliferation Induced by IL‑6 and Neutralization by Human IL‑6 Antibody. Recombinant Human IL‑6 (Catalog # 206-IL) stimulates proliferation in the T1165.85.2.1 mouse plasmacytoma cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL‑6 (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Primate
IL‑6 Monoclonal Antibody (Catalog # MAB206). The ND50 is typically 0.05-0.15 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-6

Interleukin 6 (IL-6) is a pleiotropic alpha -helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22-28 kDa phosphorylated and variably glycosylated molecule (1-4). Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6 (5). Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties (6-9). Human IL-6 is equally active on mouse and rat cells (10). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization (11). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (12). Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R (3).
Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes (3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor (4, 13).

References
  1. Van Snick, J. (1990) Annu. Rev. Immunol. 8:253.
  2. Hodge, D.R. et al. (2005) Eur. J. Cancer 41:2502.
  3. Jones, S.A. (2005) J. Immunol. 175:3468.
  4. Rose-John, S. et al. (2006) J. Leukoc. Biol. 80:227.
  5. Hirano, T. et al. (1986) Nature 324:73.
  6. Alberti, L. et al. (2005) Cancer Res. 65:2.
  7. Kestler, D.P. et al. (1995) Blood 86:4559.
  8. Kestler, D.P. et al. (1999) Am. J. Hematol. 61:169.
  9. Bihl, M.P. et al. (2002) Am. J. Respir. Cell Mol. Biol. 27:48.
  10. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099.
  11. Murakami, M. et al. (1993) Science 260:1808.
  12. Muller-Newen, G. (2003) Sci. STKE 2003:PE40.
  13. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125. 
Long Name
Interleukin 6
Entrez Gene IDs
3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 280826 (Bovine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 463288 (Primate); 100008733 (Rabbit)
Alternate Names
B cell stimulatory factor-2; B-cell differentiation factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma growth factor; IFN-beta-2; IL6; IL-6; IL-6B-cell stimulatory factor 2; Interferon beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6; MGI-2A

Product Datasheets

Citations for Human/Primate IL-6 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

57 Citations: Showing 1 - 10
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  1. Inhibition of IRE1 RNase activity modulates the tumor cell secretome and enhances response to chemotherapy
    Authors: SE Logue, EP McGrath, P Cleary, S Greene, K Mnich, A Almanza, E Chevet, RM Dwyer, A Oommen, P Legembre, F Godey, EC Madden, B Leuzzi, J Obacz, Q Zeng, JB Patterson, R Jäger, AM Gorman, A Samali
    Nat Commun, 2018;9(1):3267.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  2. Reactive astrocytic S1P3 signaling modulates the blood-tumor barrier in brain metastases
    Authors: B Gril, AN Paranjape, S Woditschka, E Hua, EL Dolan, J Hanson, X Wu, W Kloc, E Izycka-Swi, R Duchnowska, R P?ksa, W Biernat, J Jassem, N Nayyar, PK Brastianos, OM Hall, CJ Peer, WD Figg, GT Pauly, C Robinson, S Difilippan, E Bialecki, P Metellus, JP Schneider, PS Steeg
    Psychoneuroendocrinology, 2018;9(1):2705.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  3. Human central nervous system astrocytes support survival and activation of B cells: implications for MS pathogenesis
    Authors: H Touil, A Kobert, N Lebeurrier, A Rieger, P Saikali, C Lambert, L Fawaz, CS Moore, A Prat, J Gommerman, JP Antel, Y Itoyama, I Nakashima, A Bar-Or,
    J Neuroinflammation, 2018;15(1):114.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  4. Age-Related Changes to Human Tear Composition
    Authors: A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini
    Invest. Ophthalmol. Vis. Sci., 2018;59(5):2024-2031.
    Species: Human
    Sample Types: Tear
  5. The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies
    Authors: C Rolvering, AD Zimmer, A Ginolhac, C Margue, M Kirchmeyer, F Servais, HM Hermanns, S Hergovits, PV Nazarov, N Nicot, S Kreis, S Haan, I Behrmann, C Haan
    J. Leukoc. Biol., 2018;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  6. Cytosolic DNA Promotes Signal Transducer and Activator of Transcription 3 (STAT3) Phosphorylation by TANK-binding kinase 1 (TBK1) to Restrain STAT3 Activity
    Authors: HC Hsia, JE Hutti, AS Baldwin
    J. Biol. Chem, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  7. Epstein-Barr virus-induced gene 3 (EBI3) can mediate IL-6 trans-signaling
    Authors: S Chehboun, J Labrecque-, S Pasquin, Y Meliani, B Meddah, W Ferlin, M Sharma, A Tormo, JF Masson, JF Gauchat
    J. Biol. Chem, 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neut
  8. Caspase-8 Acts in a Non-enzymatic Role as a Scaffold for Assembly of a Pro-inflammatory FADDosome Complex upon TRAIL Stimulation
    Authors: CM Henry, SJ Martin
    Mol. Cell, 2017;65(4):715-729.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  9. Interleukin?6 induces an epithelial?mesenchymal transition phenotype in human adamantinomatous craniopharyngioma cells and promotes tumor cell migration
    Authors: J Zhou, C Zhang, J Pan, L Chen, ST Qi
    Mol Med Rep, 2017;15(6):4123-4131.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  10. Maslinic acid suppresses the growth of human gastric cells by inducing apoptosis via inhibition of the interleukin-6 mediated Janus kinase/signal transducer and activator of transcription 3 signaling pathway
    Authors: D Wang, S Tang, Q Zhang
    Oncol Lett, 2017;13(6):4875-4881.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  11. Monocyte Subsets Coregulate Inflammatory Responses by Integrated Signaling through TNF and IL-6 at the Endothelial Cell Interface
    Authors: M Chimen, CM Yates, HM McGettrick, LS Ward, MJ Harrison, B Apta, LH Dib, BA Imhof, P Harrison, GB Nash, GE Rainger
    J. Immunol, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  12. Tumor-Derived Mesenchymal Stem Cells Use Distinct Mechanisms to Block the Activity of Natural Killer Cell Subsets
    Authors: S Galland, J Vuille, P Martin, I Letovanec, A Caignard, G Fregni, I Stamenkovi
    Cell Rep, 2017;20(12):2891-2905.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  13. Bone metastasis of prostate cancer can be therapeutically targeted at the TBX2-WNT signaling axis
    Authors: S Nandana, M Tripathi, P Duan, CY Chu, R Mishra, C Liu, R Jin, H Yamashita, M Zayzafoon, NA Bhowmick, HE Zhau, RJ Matusik, LW Chung
    Cancer Res, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  14. Interleukin-10 and prostaglandin E2 have complementary but distinct suppressive effects on Toll-like receptor-mediated dendritic cell activation in ovarian carcinoma
    Authors: E Brencicova, AL Jagger, HG Evans, M Georgouli, A Laios, S Attard Mon, G Mehra, J Spencer, AA Ahmed, S Raju-Kanki, LS Taams, SS Diebold
    PLoS ONE, 2017;12(4):e0175712.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  15. Sensitization of EGFR wild-type non-small cell lung cancer cells to EGFR-tyrosine kinase inhibitor erlotinib
    Authors: J Raimbourg, MP Joalland, M Cabart, L de Plater, F Bouquet, A Savina, D Decaudin, J Bennouna, FM Vallette, L Lalier
    Mol. Cancer Ther., 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  16. Adipogenic differentiation of MSC alters their immunomodulatory properties in a tissue-specific manner
    Authors: H Munir, LS Ward, L Sheriff, S Kemble, S Nayar, F Barone, GB Nash, HM McGettrick
    Stem Cells, 2017;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  17. Senescence-associated IL-6 and IL-8 cytokines induce a self- and cross-reinforced senescence/inflammatory milieu strengthening tumorigenic capabilities in the MCF-7 breast cancer cell line
    Authors: P Ortiz-Mont, A Londoño-Va, JP Vernot
    Cell Commun. Signal, 2017;15(1):17.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  18. IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3
    Authors: R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie
    Cell. Physiol. Biochem., 2017;42(5):1961-1972.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Capture
  19. IL-6 trans-signaling is another pathway to upregulate Osteopontin
    Cytokine, 2016;90(0):88-95.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  20. Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium
    PLoS ONE, 2016;11(5):e0155161.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  21. Interleukin-1 Receptor Type 2 Acts with c-Fos to Enhance the Expression of Interleukin-6 and Vascular Endothelial Growth Factor A in Colon Cancer Cells and Induce Angiogenesis.
    Authors: Mar A, Chu C, Lee H, Chien C, Cheng J, Yang S, Jiang J, Lee T
    J Biol Chem, 2015;290(36):22212-24.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neut
  22. Urocortin 2 role in placental and myometrial inflammatory mechanisms at parturition.
    Authors: Voltolini C, Battersby S, Novembri R, Torricelli M, Severi F, Petraglia F, Norman J
    Endocrinology, 2015;156(2):670-9.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Capture
  23. Chemotherapy-Derived Inflammatory Responses Accelerate the Formation of Immunosuppressive Myeloid Cells in the Tissue Microenvironment of Human Pancreatic Cancer.
    Authors: Takeuchi S, Baghdadi M, Tsuchikawa T, Wada H, Nakamura T, Abe H, Nakanishi S, Usui Y, Higuchi K, Takahashi M, Inoko K, Sato S, Takano H, Shichinohe T, Seino K, Hirano S
    Cancer Res, 2015;75(13):2629-40.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Neut
  24. CXCL8 and CCL20 Enhance Osteoclastogenesis via Modulation of Cytokine Production by Human Primary Osteoblasts.
    Authors: Pathak J, Bakker A, Verschueren P, Lems W, Luyten F, Klein-Nulend J, Bravenboer N
    PLoS ONE, 2015;10(6):e0131041.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  25. Secreted ectodomain of sialic acid-binding Ig-like lectin-9 and monocyte chemoattractant protein-1 promote recovery after rat spinal cord injury by altering macrophage polarity.
    Authors: Matsubara K, Matsushita Y, Sakai K, Kano F, Kondo M, Noda M, Hashimoto N, Imagama S, Ishiguro N, Suzumura A, Ueda M, Furukawa K, Yamamoto A
    J Neurosci, 2015;35(6):2452-64.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Neut
  26. Digital microfluidic assay for protein detection.
    Authors: Mok J, Mindrinos M, Davis R, Javanmard M
    Proc Natl Acad Sci U S A, 2014;111(6):2110-5.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Microfluidic Assay
  27. Pancreatic cancer-associated stellate cells promote differentiation of myeloid-derived suppressor cells in a STAT3-dependent manner.
    Authors: Mace T, Ameen Z, Collins A, Wojcik S, Mair M, Young G, Fuchs J, Eubank T, Frankel W, Bekaii-Saab T, Bloomston M, Lesinski G
    Cancer Res, 2014;73(10):3007-18.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  28. Epstein-Barr virus infection induces indoleamine 2,3-dioxygenase expression in human monocyte-derived macrophages through p38/mitogen-activated protein kinase and NF-kappaB pathways: impairment in T cell functions.
    Authors: Liu W, Lin Y, Xiao H, Xing S, Chen H, Chi P, Zhang G
    J Virol, 2014;88(12):6660-71.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  29. Podocytes regulate neutrophil recruitment by glomerular endothelial cells via IL-6-mediated crosstalk.
    Authors: Kuravi S, McGettrick H, Satchell S, Saleem M, Harper L, Williams J, Rainger G, Savage C
    J Immunol, 2014;193(1):234-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  30. Reference range and short- and long-term biological variation of interleukin (IL)-6, IL-17A and tissue necrosis factor-alpha using high sensitivity assays.
    Authors: Todd J, Simpson P, Estis J, Torres V, Wub A
    Cytokine, 2013;64(3):660-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  31. Pre-analytical effects of blood sampling and handling in quantitative immunoassays for rheumatoid arthritis.
    Authors: Zhao X, Qureshi F, Eastman PS, Manning WC, Alexander C, Robinson WH, Hesterberg LK
    J. Immunol. Methods, 2012;378(1):72-80.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  32. Monocytes Induce STAT3 Activation in Human Mesenchymal Stem Cells to Promote Osteoblast Formation.
    Authors: Nicolaidou V, Wong MM, Redpath AN
    PLoS ONE, 2012;7(7):e39871.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  33. Protection from RNA and DNA viruses by IL-32.
    Authors: Zepp JA, Nold-Petry CA, Dinarello CA, Nold MF
    J. Immunol., 2011;186(7):4110-8.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescence
  34. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010;28(5):939-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  35. TLR2-activated human langerhans cells promote Th17 polarization via IL-1beta, TGF-beta and IL-23.
    Authors: Aliahmadi E, Gramlich R, Grutzkau A, Hitzler M, Kruger M, Baumgrass R, Schreiner M, Wittig B, Wanner R, Peiser M
    Eur. J. Immunol., 2009;39(5):1221-30.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  36. Noninvasive detection of acute and chronic injuries in human renal transplant by elevation of multiple cytokines/chemokines in urine.
    Authors: Hu H, Kwun J, Aizenstein BD, Knechtle SJ
    Transplantation, 2009;87(12):1814-20.
    Species: Human
    Sample Types: Urine
    Applications: Antibody Array Development
  37. Resistance of human alveolar macrophages to Bacillus anthracis lethal toxin.
    Authors: Wu W, Mehta H, Chakrabarty K, Booth JL, Duggan ES, Patel KB, Ballard JD, Coggeshall KM, Metcalf JP
    J. Immunol., 2009;183(9):5799-806.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  38. Maternal and cord plasma cytokine and chemokine profile in pregnancies complicated by asthma.
    Authors: Osei-Kumah A, Smith R, Clifton VL
    Cytokine, 2008;43(2):187-93.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  39. Lipopolysaccharide-squamous cell carcinoma-monocyte interactions induce cancer-supporting factors leading to rapid STAT3 activation.
    Authors: Kurago ZB, Lam-ubol A, Stetsenko A
    Head Neck Pathol, 2008;2(1):1-12.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  40. Bacterial vaginosis, not HIV, is primarily responsible for increased vaginal concentrations of proinflammatory cytokines.
    Authors: Mitchell CM, Balkus J, Agnew KJ, Cohn S, Luque A, Lawler R, Coombs RW, Hitti JE
    AIDS Res. Hum. Retroviruses, 2008;24(5):667-71.
    Species: Human
    Sample Types: Vaginal Fluid
    Applications: ELISA Development
  41. Endogenous IL-32 controls cytokine and HIV-1 production.
    Authors: Nold MF, Nold-Petry CA, Pott GB, Zepp JA, Saavedra MT, Kim SH, Dinarello CA
    J. Immunol., 2008;181(1):557-65.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Electrochemiluminescence
  42. Human airway smooth muscle promotes human lung mast cell survival, proliferation, and constitutive activation: cooperative roles for CADM1, stem cell factor, and IL-6.
    Authors: Hollins F, Kaur D, Yang W, Cruse G, Saunders R, Sutcliffe A, Berger P, Ito A, Brightling CE, Bradding P
    J. Immunol., 2008;181(4):2772-80.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  43. C5a and TNF-alpha up-regulate the expression of tissue factor in intra-alveolar neutrophils of patients with the acute respiratory distress syndrome.
    Authors: Kambas K, Markiewski MM, Pneumatikos IA, Rafail SS, Theodorou V, Konstantonis D, Kourtzelis I, Doumas MN, Magotti P, Deangelis RA, Lambris JD, Ritis KD
    J. Immunol., 2008;180(11):7368-75.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  44. Tumor necrosis factor alpha as a new target for renal cell carcinoma: two sequential phase II trials of infliximab at standard and high dose.
    Authors: Harrison ML, Obermueller E, Maisey NR, Hoare S, Edmonds K, Li NF, Chao D, Hall K, Lee C, Timotheadou E, Charles K, Ahern R, King DM, Eisen T, Corringham R, DeWitte M, Balkwill F, Gore M
    J. Clin. Oncol., 2007;25(29):4542-9.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC Frozen
  45. Ultrasensitive flow-based immunoassays using single-molecule counting.
    Authors: Todd J, Freese B, Lu A, Held D, Morey J, Livingston R, Goix P
    Clin. Chem., 2007;53(11):1990-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  46. Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
    Authors: Arnold R, Neumann M, Konig W
    Immunology, 2007;121(1):71-81.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  47. Toll-like receptors 3 and 4 are expressed by human bone marrow-derived mesenchymal stem cells and can inhibit their T-cell modulatory activity by impairing Notch signaling.
    Authors: Liotta F, Angeli R, Cosmi L, Fili L, Manuelli C, Frosali F, Mazzinghi B, Maggi L, Pasini A, Lisi V, Santarlasci V, Consoloni L, Angelotti ML, Romagnani P, Parronchi P, Krampera M, Maggi E, Romagnani S, Annunziato F
    Stem Cells, 2007;26(1):279-89.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  48. Th2 cytokine response in Major Depressive Disorder patients before treatment.
    Authors: Pavon L, Kiryushko D, Novitskaya V, Sandoval-Lopez G, Eugenia Hernandez M, Soroka V, Klingelhofer J, Loria F, Estrada I, Lukanidin E, Berezin V, Perez M, Moreno J, Bock E, Avila U, Leff P, Anton B, Heinze G
    J. Neuroimmunol., 2006;172(1):156-65.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development
  49. Differential survival of leukocyte subsets mediated by synovial, bone marrow, and skin fibroblasts: site-specific versus activation-dependent survival of T cells and neutrophils.
    Authors: Filer A, Parsonage G, Smith E, Osborne C, Thomas AM, Curnow SJ, Rainger GE, Raza K, Nash GB, Lord J, Salmon M, Buckley CD
    Arthritis Rheum., 2006;54(7):2096-108.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neut
  50. Interleukin-6 and interleukin-8 in cervical fluid in a population of Swedish women in preterm labor: relationship to microbial invasion of the amniotic fluid, intra-amniotic inflammation, and preterm delivery.
    Authors: Holst RM, Mattsby-Baltzer I, Wennerholm UB, Hagberg H, Jacobsson B
    Acta Obstet Gynecol Scand, 2005;84(6):551-7.
    Species: Human
    Sample Types: Amniotic Fluid
    Applications: ELISA Development
  51. Rapid analysis of inflammatory cytokines in cerebrospinal fluid using chip-based immunoaffinity electrophoresis.
    Authors: Phillips TM
    Electrophoresis, 2004;25(10):1652-9.
    Species: Human
    Sample Types: Serum
    Applications: Affinity Chromatography
  52. trans fatty acids and systemic inflammation in heart failure.
    Authors: Mozaffarian D, Rimm EB, King IB, Lawler RL, McDonald GB, Levy WC
    Am. J. Clin. Nutr., 2004;80(6):1521-5.
    Species: Human
    Sample Types: Plasma
    Applications: ELISA Development
  53. Direct comparison of traditional ELISAs and membrane protein arrays for detection and quantification of human cytokines.
    Authors: Copeland S, Siddiqui J, Remick D
    J. Immunol. Methods, 2004;284(1):99-106.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Array Development
  54. Interplay between IFN-gamma and IL-6 signaling governs neutrophil trafficking and apoptosis during acute inflammation.
    Authors: McLoughlin RM, Witowski J, Robson RL, Wilkinson TS, Hurst SM, Williams AS, Williams JD, Rose-John S, Jones SA, Topley N
    J. Clin. Invest., 2003;112(4):598-607.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  55. Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells.
    Authors: Hurst SM, McLoughlin RM, Monslow J, Owens S, Morgan L, Fuller GM, Topley N, Jones SA
    J. Immunol., 2002;169(9):5244-51.
    Species: Human
    Sample Types: Peritoneal Fluid
    Applications: ELISA Development
  56. The reprogrammed host: Chlamydia trachomatis-induced up-regulation of glycoprotein 130 cytokines, transcription factors, and antiapoptotic genes.
    Authors: Hess S, Rheinheimer C, Tidow F, Bartling G, Kaps C, Lauber J, Buer J, Klos A
    Arthritis Rheum., 2001;44(10):2392-401.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: ELISA Development
  57. Measurement of interleukin-33 (IL-33) and IL-33 receptors (sST2 and ST2L) in patients with rheumatoid arthritis.
    Authors: Hong YS, Moon S, Moon SJ, Joo YB, Joo Y, Jeon C, Jeon CH, Cho ML, Ju JH, Oh HJ, Heo YJ, Heo Y, Park SH, Kim HY, Min JK
    J Korean Med Sci, 0;26(9):1132-9.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development

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Reviews for Human/Primate IL-6 Antibody

Average Rating: 4.4 (Based on 10 Reviews)

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Human/Primate IL-6 Antibody
By Anonymous on 12/19/2018
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human/Primate IL-6 Antibody
By Anonymous on 11/20/2018
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Human/Primate IL-6 Antibody
By Anonymous on 11/07/2018
Application: Microarrays Sample Tested: EDTA Plasma Species: Human

Human/Primate IL-6 Antibody
By Anonymous on 11/01/2018
Application: MSD assay Sample Tested: Vitreous Humor Species: Cynomolgus Monkey

After biotinylation, used as a capture reagent according to the manufacturer’s protocol (Meso Scale Diagnostics LLC). Paired with goat PC IL-6 antibody (AF-206-NA) as a detection antibody. A standard curve with recombinant human IL-6 (Cat# 206-IL/CF) is shown (1.6-25,000 pg/ml).


Human/Primate IL-6 Antibody
By Anonymous on 04/24/2018
Application: IHC Sample Tested: Spleen tissue Species: Human

Human/Primate IL-6 Antibody
By Anonymous on 04/13/2018
Application: WB Sample Tested: Tumor cell lyastes Species: Human

Human/Primate IL-6 Antibody
By Anonymous on 03/30/2018
Application: ICC/IF Sample Tested: Human affected ileum tissue from Crohn's patients Species: Human ileum tissue,Human normal resection margin tissue from Crohn's patients

Li C, Iness A, Yoon J, Grider JR, Murthy KS, Kellum JM, Kuemmerle JF. Increased autocrine IL-6 and STAT3 (Ser727) phosphorylation is profibrotic regulating TGF-β1 and Collagen I in muscle of stricturing Crohn's disease. J Immunol. 194(7):3422-31; 2015


Human/Primate IL‑6 Antibody
By Anonymous on 03/23/2018
Application: WB Sample Tested: Human recombinant test antibody Species: Human

Human/Primate IL-6 Antibody
By Anonymous on 05/16/2017
Application: Func Sample Tested: Kidney cancer cells Species: Human

Human/Primate IL-6 Antibody
By Anonymous on 04/26/2017
Application: B/N Sample Tested: Neutrophils Species: Human

IL-6 secretion was neutralized in neutrophils and its effect on tumor cell extravasation was assessed.