Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all BNP Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Pro-BNP
His27-His134
Accession # P16860

Specificity

Detects human Pro-BNP in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Pro‑BNP Antibody

Detection of Human Pro-BNP antibody by Western Blot.

Detection of Human Pro‑BNP by Western Blot.

Western blot shows lysates of human plasma. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pro-BNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3604) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pro-BNP at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Pro‑BNP Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Human plasma

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BNP

Human BNP (brain natriuretic peptide) is a 3.5 kDa, secreted, nonglycosylated peptide that belongs to the natriuretic peptide family. It is the product of ventricular myocytes and bone marrow endothelial cells. BNP is synthesized as a 12 kDa, 108 amino acid (aa) prohormone with a 76 aa prosegment and a 32 aa mature (BNP) region. Both forms circulate, with the prohormone showing O-linked glycosylation that generates a 25-27 kDa glycoprotein. Glycosylation is suggested to stabilize the molecule. The proform also tends to noncovalently trimerize. In blood, 1-32 BNP is proteolytically processed into 3-32 BNP, with loss of Ser-Pro at the N-terminus. Human Pro-BNP is 29% and 50% aa identical to mouse and pig Pro-BNP, respectively.

Long Name

Brain Natriuretic Peptide

Alternate Names

BNF, NPPB

Entrez Gene IDs

4879 (Human); 18158 (Mouse); 25105 (Rat)

Gene Symbol

NPPB

UniProt

P16860

Additional BNP Products

Product Documents for Human Pro‑BNP Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Pro‑BNP Antibody

For research use only

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