|Detection of Human Pro‑BNP by Western Blot. Western blot shows lysates of human plasma. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human Pro‑BNP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3604) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Pro‑BNP at approximately 27 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.|
Human BNP (brain natriuretic peptide) is a 3.5 kDa, secreted, nonglycosylated peptide that belongs to the natriuretic peptide family. It is the product of ventricular myocytes and bone marrow endothelial cells. BNP is synthesized as a 12 kDa, 108 amino acid (aa) prohormone with a 76 aa prosegment and a 32 aa mature (BNP) region. Both forms circulate, with the prohormone showing O-linked glycosylation that generates a 25-27 kDa glycoprotein. Glycosylation is suggested to stabilize the molecule. The proform also tends to noncovalently trimerize. In blood, 1-32 BNP is proteolytically processed into 3-32 BNP, with loss of Ser-Pro at the N-terminus. Human Pro-BNP is 29% and 50% aa identical to mouse and pig Pro-BNP, respectively.
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