Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Xenograft

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Prolyl Oligopeptidase/PREP Primary Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PREP
Leu2-Pro710
Accession # P48147

Specificity

Detects human PREP in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse PREP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human Prolyl Oligopeptidase/PREP Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Cell lysates spiked with Recombinant Human Prolyl Oligopeptidase/PREP (Catalog # 4308‑SE), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Prolyl Oligopeptidase/PREP (Catalog # 4308-SE)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Prolyl Oligopeptidase/PREP

Prolyl Oligopeptidase is a serine peptidase displaying specificity for the cleavage of Pro-Xaa bonds of oligopeptide substrates (1, 2). The peptidase is known to hydrolyze a variety of biologically active peptides such as bradykinin, substance P, neurotensin, and vasopressin (3). Because of its action on neuropeptides, Prolyl Oligopeptidase is considered to be involved in processes such as learning, memory, and depression (4).

References

  1. Tarrago, T. et al. (2005) J. Pept. Sci. 11:283.
  2. Yoshimoto, T. et al. (1977) Biochemistry. 16:2942.
  3. Wilk, S. (1983) Life Sci. 33:2149.
  4. Maes, M. et al. (1994) Biol. Psychiatry. 35:545.

Alternate Names

PE, PREP, rPop

Entrez Gene IDs

5550 (Human); 19072 (Mouse); 83471 (Rat)

Gene Symbol

PREP

UniProt

P48147

Additional Prolyl Oligopeptidase/PREP Products

Product Documents for Human Prolyl Oligopeptidase/PREP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Prolyl Oligopeptidase/PREP Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Human Prolyl Oligopeptidase/PREP Antibody

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