Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 391609
View all Protein S/PROS1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Protein S
Ala42-Trp670
Accession # P07225

Specificity

Detects human Protein S in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross‑react with recombinant mouse Protein S.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Protein S/PROS1 Antibody

Detection of Human Protein S/PROS1 antibody by Western Blot.

Detection of Human Protein S/PROS1 by Western Blot.

Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4036) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Protein S/PROS1 at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Protein S/PROS1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample:   Human plasma under non-reducing conditions only

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protein S/PROS1

The PROS1 gene encodes Protein S (PS), a ~70 kDa vitamin-K dependent plasma glycoprotein mainly synthesized by human hepatocytes. PS is a non-enzymatic cofactor in the degradation of coagulation factors Va and VIIIa and thus plays an anticoagulant role. Approximately 60% circulates in complex with C4b-binding protein. Free PS is a mixture of monomers and multimers. The 652 aa PS proprotein is heavily modified with 11 carboxyglutamates, one hydroxyaspartate, disulfides and glycosylation. Human Protein S shows 80% aa identity with mouse Protein S.

Alternate Names

PROS1

Entrez Gene IDs

5627 (Human); 19128 (Mouse); 81750 (Rat)

Gene Symbol

PROS1

UniProt

P07225

Additional Protein S/PROS1 Products

Product Documents for Human Protein S/PROS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Protein S/PROS1 Antibody

For research use only

Citations for Human Protein S/PROS1 Antibody

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