Detection of Human Protein S/PROS1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Protein S/PROS1 Monoclonal Antibody (Catalog # MAB4036) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Protein S/PROS1 at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protein S/PROS1
The PROS1 gene encodes Protein S (PS), a ~70 kDa vitamin-K dependent
plasma glycoprotein mainly synthesized by human hepatocytes. PS is a
non-enzymatic cofactor in the degradation of coagulation factors Va and
VIIIa and thus plays an anticoagulant role. Approximately 60%
circulates in complex with C4b-binding protein. Free PS is a mixture of
monomers and multimers. The 652 aa PS proprotein is heavily modified
with 11 carboxyglutamates, one hydroxyaspartate, disulfides and
glycosylation. Human Protein S shows 80% aa identity with mouse Protein
Entrez Gene IDs:
5627 (Human); 19128 (Mouse); 81750 (Rat)
EC 3.4.21; PROS; PROS1; protein S (alpha); protein Sa; PS21; PS22; PS23; PS24; PS25; PSA; vitamin K-dependent plasma protein S; vitamin K-dependent protein S
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