Human Protocadherin gamma C3 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Protocadherin gamma C3 in direct ELISAs. Stains human Protocadherin gamma C3 transfectants but not irrelevant transfectants in flow cytometry.
  • Source
    Monoclonal Mouse IgG1 Clone # 926518
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin gamma C3
    Met1-Tyr693
    Accession # Q9UN70
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Protocadherin gamma C3 in HEK293 Human Cell Line Transfected with Human Protocadherin gamma C3 by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either human Protocadherin gamma C3 (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human Protocadherin gamma C3 Monoclonal Antibody (Catalog # MAB83641), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Protocadherin gamma C3
Protocadherin  gamma  C3 is a member of the gamma  subgroup of clustered protocadherins (1). Like other gamma  protocadherins, mature Protocadherin  gamma  C3 contains six extracellular cadherin domains, a transmembrane region, and a cytoplasmic domain (2, 3). Within the ECD, human Protocadherin  gamma  C3 shares 91% and 92% amino acid sequence identity with mouse and rat Protocadherin gamma C3, respectively. It plays an important role in cell adhesion and cell recognition through CA2+ -dependent homophilic interaction (4). MMP-mediated shedding of gamma  protocadherins and release of their cytoplasmic domain by the gamma -secretase complex results in translocation of the intracellular domain into the nucleus and transcriptional activation of target genes (5-7). Protocadherin  gamma  C3 is cleaved within its ectodomain by ADAM10 in fibroblasts and neuronal cells (8). Deletion of the entire protocadherin gamma  gene cluster is embryonic lethal in mice (9). Protocadherin  gamma  C3 is most notably expressed in the nervous system (10). Conditional deletion of the protocadherin  gamma  gene cluster in mice affects development of retinal ganglion cells and spinal cord interneurons, resulting in decreased synapses and increased neuronal apoptosis (9, 11-14). The C-type protocadherin gamma  isoforms specifically may be responsible for the increased apoptosis observed in mice lacking the entire protocadherin gamma  gene cluster (15). Cortical neuron-specific deletion of the protocadherin gamma  gene cluster results in dendritic arborization defects (16). The protocadherin gamma  subfamily may also be involved in cerebrospinal fluid production and the maturation and differentiation of postnatally born olfactory granule cells (17, 18).
  • References:
    1. Yagi, T. (2008) Dev. Growth Differ. 50:S131.
    2. Sano, K. et al. (1993) EMBO J. 12:2249.
    3. Kohmura, N. et al. (1998) Neuron 20:1137.
    4. Obata, S. et al. (1995) J. Cell Sci. 108:3765.
    5. Hambsch, B. et al. (2005) J. Biol. Chem. 280:15888.
    6. Haas, I.G. et al. (2005) J. Biol. Chem. 280:9313.
    7. Bonn, S. et al. (2007) Mol. Cell. Biol. 27:4121.
    8. Reiss, K. et al. (2006) J. Biol. Chem. 281:21735.
    9. Wang, X. et al. (2002) Neuron 36:843.
    10. Phillips, G.R. et al. (2003) J. Neurosci. 23:5096.
    11. Weiner, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:8.
    12. Lefebvre, J.L. et al. (2008) Development 135:4141.
    13. Prasad, T. et al. (2008) Development 135:4153.
    14. Lin, C. et al. (2010) J. Biol. Chem. 285:41675.
    15. Chen, W.V. et al. (2012) Neuron 75:402.
    16. Garrett, A.M. et al. (2012) Neuron 74:269.
    17. Lobas, M.A. et al. (2012) J. Neurochem. 120:913.
    18. Ledderose, J. et al. (2013) Sci. Rep. 3:1514.
  • Entrez Gene IDs:
    5098 (Human); 93706 (Mouse); 116782 (Rat)
  • Alternate Names:
    Cadherin-Like 2; PC43; PC-43; PCDH2; PCDH-GAMMA-C3; PCDHGC3; protocadherin 2; Protocadherin 43; Protocadherin gamma C3; protocadherin gamma subfamily C, 3; protocadherin gamma-C3; Protocadherin-2; protocadherin-43
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Secondary Antibodies
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

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Mouse IgG Antibody

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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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Mouse IgG VisUCyte HRP Polymer Antibody

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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

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