Human Protocadherin gamma C3 Antibody

Catalog # Availability Size / Price Qty
MAB8364
MAB8364-SP
Detection of Human Protocadherin  gamma  C3 by Western Blot.
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Human Protocadherin gamma C3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Protocadherin gamma C3 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 926525
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin gamma C3
Met1-Tyr693
Accession # Q9UN70
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Western Blot Detection of Human Protocadherin  gamma  C3 by Western Blot. View Larger

Detection of Human Protocadherin gamma C3 by Western Blot. Western blot shows lysates of H4 human neuroglioma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Protocadherin gamma C3 Monoclonal Antibody (Catalog # MAB8364) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Protocadherin  gamma  C3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Protocadherin gamma C3

Protocadherin  gamma  C3 is a member of the gamma  subgroup of clustered protocadherins (1). Like other gamma  protocadherins, mature Protocadherin  gamma  C3 contains six extracellular cadherin domains, a transmembrane region, and a cytoplasmic domain (2, 3). Within the ECD, human Protocadherin  gamma  C3 shares 91% and 92% amino acid sequence identity with mouse and rat Protocadherin gamma C3, respectively. It plays an important role in cell adhesion and cell recognition through CA2+ -dependent homophilic interaction (4). MMP-mediated shedding of gamma  protocadherins and release of their cytoplasmic domain by the gamma -secretase complex results in translocation of the intracellular domain into the nucleus and transcriptional activation of target genes (5-7). Protocadherin  gamma  C3 is cleaved within its ectodomain by ADAM10 in fibroblasts and neuronal cells (8). Deletion of the entire protocadherin gamma  gene cluster is embryonic lethal in mice (9). Protocadherin  gamma  C3 is most notably expressed in the nervous system (10). Conditional deletion of the protocadherin  gamma  gene cluster in mice affects development of retinal ganglion cells and spinal cord interneurons, resulting in decreased synapses and increased neuronal apoptosis (9, 11-14). The C-type protocadherin gamma  isoforms specifically may be responsible for the increased apoptosis observed in mice lacking the entire protocadherin gamma  gene cluster (15). Cortical neuron-specific deletion of the protocadherin gamma  gene cluster results in dendritic arborization defects (16). The protocadherin gamma  subfamily may also be involved in cerebrospinal fluid production and the maturation and differentiation of postnatally born olfactory granule cells (17, 18).

References
  1. Yagi, T. (2008) Dev. Growth Differ. 50:S131.
  2. Sano, K. et al. (1993) EMBO J. 12:2249.
  3. Kohmura, N. et al. (1998) Neuron 20:1137.
  4. Obata, S. et al. (1995) J. Cell Sci. 108:3765.
  5. Hambsch, B. et al. (2005) J. Biol. Chem. 280:15888.
  6. Haas, I.G. et al. (2005) J. Biol. Chem. 280:9313.
  7. Bonn, S. et al. (2007) Mol. Cell. Biol. 27:4121.
  8. Reiss, K. et al. (2006) J. Biol. Chem. 281:21735.
  9. Wang, X. et al. (2002) Neuron 36:843.
  10. Phillips, G.R. et al. (2003) J. Neurosci. 23:5096.
  11. Weiner, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:8.
  12. Lefebvre, J.L. et al. (2008) Development 135:4141.
  13. Prasad, T. et al. (2008) Development 135:4153.
  14. Lin, C. et al. (2010) J. Biol. Chem. 285:41675.
  15. Chen, W.V. et al. (2012) Neuron 75:402.
  16. Garrett, A.M. et al. (2012) Neuron 74:269.
  17. Lobas, M.A. et al. (2012) J. Neurochem. 120:913.
  18. Ledderose, J. et al. (2013) Sci. Rep. 3:1514.
Entrez Gene IDs
5098 (Human); 93706 (Mouse); 116782 (Rat)
Alternate Names
Cadherin-Like 2; PC43; PC-43; PCDH2; PCDH-GAMMA-C3; PCDHGC3; protocadherin 2; Protocadherin 43; Protocadherin gamma C3; protocadherin gamma subfamily C, 3; protocadherin gamma-C3; Protocadherin-2; protocadherin-43

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Isotype Controls

Mouse IgG1 Isotype Control

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Secondary Antibodies

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