Human Protocadherin gamma C3 Antibody (926525)

R&D Systems | Catalog # MAB8364

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 926525
View all Protocadherin gamma C3 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Protocadherin gamma C3
Met1-Tyr693
Accession # Q9UN70

Specificity

Detects human Protocadherin gamma C3 in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Protocadherin gamma C3 Antibody (926525)

Detection of Human Protocadherin ? C3 antibody by Western Blot.

Detection of Human Protocadherin gamma C3 by Western Blot.

Western blot shows lysates of H4 human neuroglioma cell line and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Protocadherin gamma C3 Monoclonal Antibody (Catalog # MAB8364) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Protocadherin gamma C3 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Protocadherin gamma C3 Antibody (926525)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: H4 human neuroglioma cell line and U‑118‑MG human glioblastoma/astrocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Protocadherin gamma C3

Protocadherin  gamma  C3 is a member of the gamma  subgroup of clustered protocadherins (1). Like other gamma  protocadherins, mature Protocadherin  gamma  C3 contains six extracellular cadherin domains, a transmembrane region, and a cytoplasmic domain (2, 3). Within the ECD, human Protocadherin  gamma  C3 shares 91% and 92% amino acid sequence identity with mouse and rat Protocadherin gamma C3, respectively. It plays an important role in cell adhesion and cell recognition through CA2+ -dependent homophilic interaction (4). MMP-mediated shedding of gamma  protocadherins and release of their cytoplasmic domain by the gamma -secretase complex results in translocation of the intracellular domain into the nucleus and transcriptional activation of target genes (5-7). Protocadherin  gamma  C3 is cleaved within its ectodomain by ADAM10 in fibroblasts and neuronal cells (8). Deletion of the entire protocadherin gamma  gene cluster is embryonic lethal in mice (9). Protocadherin  gamma  C3 is most notably expressed in the nervous system (10). Conditional deletion of the protocadherin  gamma  gene cluster in mice affects development of retinal ganglion cells and spinal cord interneurons, resulting in decreased synapses and increased neuronal apoptosis (9, 11-14). The C-type protocadherin gamma  isoforms specifically may be responsible for the increased apoptosis observed in mice lacking the entire protocadherin gamma  gene cluster (15). Cortical neuron-specific deletion of the protocadherin gamma  gene cluster results in dendritic arborization defects (16). The protocadherin gamma  subfamily may also be involved in cerebrospinal fluid production and the maturation and differentiation of postnatally born olfactory granule cells (17, 18).

References

  1. Yagi, T. (2008) Dev. Growth Differ. 50:S131.
  2. Sano, K. et al. (1993) EMBO J. 12:2249.
  3. Kohmura, N. et al. (1998) Neuron 20:1137.
  4. Obata, S. et al. (1995) J. Cell Sci. 108:3765.
  5. Hambsch, B. et al. (2005) J. Biol. Chem. 280:15888.
  6. Haas, I.G. et al. (2005) J. Biol. Chem. 280:9313.
  7. Bonn, S. et al. (2007) Mol. Cell. Biol. 27:4121.
  8. Reiss, K. et al. (2006) J. Biol. Chem. 281:21735.
  9. Wang, X. et al. (2002) Neuron 36:843.
  10. Phillips, G.R. et al. (2003) J. Neurosci. 23:5096.
  11. Weiner, J.A. et al. (2005) Proc. Natl. Acad. Sci. USA 102:8.
  12. Lefebvre, J.L. et al. (2008) Development 135:4141.
  13. Prasad, T. et al. (2008) Development 135:4153.
  14. Lin, C. et al. (2010) J. Biol. Chem. 285:41675.
  15. Chen, W.V. et al. (2012) Neuron 75:402.
  16. Garrett, A.M. et al. (2012) Neuron 74:269.
  17. Lobas, M.A. et al. (2012) J. Neurochem. 120:913.
  18. Ledderose, J. et al. (2013) Sci. Rep. 3:1514.

Alternate Names

Cadherin-Like 2, PCDH2, PCDHGC3, Protocadherin 43, Protocadherin-2

Entrez Gene IDs

5098 (Human); 93706 (Mouse); 116782 (Rat)

Gene Symbol

PCDHGC3

UniProt

Q9UN70

Additional Protocadherin gamma C3 Products

Product Documents for Human Protocadherin gamma C3 Antibody (926525)

Certificate of Analysis

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Product Specific Notices for Human Protocadherin gamma C3 Antibody (926525)

For research use only

Related Research Areas

Cadherin Superfamily

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