Human PSMB10/MECL1 Antibody

Catalog # Availability Size / Price Qty
Detection of Human PSMB10/MECL1 by Western Blot.
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Product Details
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Human PSMB10/MECL1 Antibody Summary

Species Reactivity
Detects human PSMB10/MECL1 in direct ELISAs and Western blots. In direct ELISAs, less than 6% cross-reactivity with recombinant human PSMB7 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
E. coli-derived recombinant human PSMB10/MECL1
Accession # P40306
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PSMB10/MECL1 antibody by Western Blot. View Larger

Detection of Human PSMB10/MECL1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, HuT 78 human cutaneous T cell lymphoma cell line, and THP-1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PSMB10/MECL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7684) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PSMB10/MECL1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PSMB10/MECL1

PSMB10 (Proteasome Subunit beta type-10; Also MECL1 and LMP10) is a 25-26 kDa member of the peptidase T1B family of molecules. It is expressed a wide variety of cells, particularly those responsive to proinflammatory cytokines. PSMB10 is expressed both constitutively and via IFN-gamma induction in a cell-specific manner. It contributes to the 700 kDa, 20S proteasome catalytic complex, a dynamic intracellular structure that participates in the ATP-dependent ubiquitin-proteasome-system used for protein degradation. PSMB10 qualifies as a beta -type, i (immuno)-type proteasome, meaning it both plays a catalytic role in the turnover of proteins, and is found in cytokine-responsive cells. The i-type of proteasome is believed to protect cells against the accumulation of oxidant-damaged proteins. This activity is absolutely dependent upon the removal of the precursor's prosequence, an action that exposes a critical internal Thr residue. Human PSMB10 is synthesized as a 29 kDa, 273 amino acid (aa) proprecursor. It contains a 39 aa autocleavable propeptide plus a 234 aa mature region. The mature region shows no identifiable standard structural motifs. Over aa 40-273, human PSMB10 shares 89% aa sequence identity with mouse PSMB10.

Long Name
Proteasome (Prosome, Macropain) Subunit, beta Type, 10
Entrez Gene IDs
5699 (Human); 19171 (Mouse); 291983 (Rat)
Alternate Names
beta2i; FLJ00366; LMP10; LMP10Proteasome subunit beta-2i; Low molecular mass protein 10; Macropain subunit MECl-1; MECL1; MECl-1; MECL1EC; MGC1665; Multicatalytic endopeptidase complex subunit MECl-1; proteasome (prosome, macropain) subunit, beta type, 10; proteasome catalytic subunit 2i; Proteasome MECl-1; proteasome subunit beta 7i; proteasome subunit beta type-10; Proteasome subunit beta-2i; proteasome subunit MECL1; PSMB10

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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