Human PSMB10/MECL1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PSMB10/MECL1 in direct ELISAs and Western blots. In direct ELISAs, less than 6% cross-reactivity with recombinant human PSMB7 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PSMB10/MECL1
    Thr40-Glu273
    Accession # P40306
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PSMB10/MECL1 by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, Raji human Burkitt's lymphoma cell line, HuT 78 human cutaneous T cell lymphoma cell line, and THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PSMB10/MECL1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7684) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PSMB10/MECL1 at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSMB10/MECL1

PSMB10 (Proteasome Subunit beta type-10; Also MECL1 and LMP10) is a 25-26 kDa member of the peptidase T1B family of molecules. It is expressed a wide variety of cells, particularly those responsive to proinflammatory cytokines. PSMB10 is expressed both constitutively and via IFN-gamma induction in a cell-specific manner. It contributes to the 700 kDa, 20S proteasome catalytic complex, a dynamic intracellular structure that participates in the ATP-dependent ubiquitin-proteasome-system used for protein degradation. PSMB10 qualifies as a beta -type, i (immuno)-type proteasome, meaning it both plays a catalytic role in the turnover of proteins, and is found in cytokine-responsive cells. The i-type of proteasome is believed to protect cells against the accumulation of oxidant-damaged proteins. This activity is absolutely dependent upon the removal of the precursor's prosequence, an action that exposes a critical internal Thr residue. Human PSMB10 is synthesized as a 29 kDa, 273 amino acid (aa) proprecursor. It contains a 39 aa autocleavable propeptide plus a 234 aa mature region. The mature region shows no identifiable standard structural motifs. Over aa 40-273, human PSMB10 shares 89% aa sequence identity with mouse PSMB10.

  • Long Name:
    Proteasome (Prosome, Macropain) Subunit, beta Type, 10
  • Entrez Gene IDs:
    5699 (Human); 19171 (Mouse); 291983 (Rat)
  • Alternate Names:
    beta2i; FLJ00366; LMP10; LMP10Proteasome subunit beta-2i; Low molecular mass protein 10; Macropain subunit MECl-1; MECL1; MECl-1; MECL1EC 3.4.25.1; MGC1665; Multicatalytic endopeptidase complex subunit MECl-1; proteasome (prosome, macropain) subunit, beta type, 10; proteasome catalytic subunit 2i; Proteasome MECl-1; proteasome subunit beta 7i; proteasome subunit beta type-10; Proteasome subunit beta-2i; proteasome subunit MECL1; PSMB10
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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