Human PSP94 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human PSP94 in direct ELISAs and Western blots.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant human PSP94 isoform 1 (R&D Systems, Catalog # 3780-PS)
    Ser21-Ile114
    Accession # P08118
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    10 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human PSP94/MSMB by Simple WesternTM. Simple Western lane view shows lysates of human prostate tissue, loaded at 0.2 mg/mL. A specific band was detected for PSP94/MSMB at approximately 17 kDa (as indicated) using 10 µg/mL of Goat Anti-Human PSP94/MSMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3780) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system.
Detection of Human PSP94/MSMB by Western Blot. Western blot shows lysates of human prostate tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human PSP94/MSMB Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3780) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for PSP94/MSMB at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PSP94/MSMB

PSP94 (prostate secretory protein of 94 amino acids; also named  beta -MSP) is a secreted, non-glycosylated member of the beta -microseminoprotein family (1). The 94 amino acid (aa) mature PSP94 contains no classic motifs or domains, but does have ten Cys that are conserved across species (1, 2). It is expressed in mucoid secretions, but its function is unknown (2, 3). PSP94 is abundant in prostatic fluid, which is the exclusive source in rodents (4). Gastric and respiratory secretory epithelia are also significant sources in humans (2, 3). Human PSP94 circulates bound to a 71 kDa PSP binding protein, possibly disulfide-linked (5). The seminal fluid protein CRISP-3 can also bind PSP94 (6). PSP94 has been proposed as an alternative to PSA as a serum marker for prostate cancer. When total (bound plus free) PSP94 is considered, its secretion is found to be down-regulated in cancer cells, creating below normal circulating levels (7, 8). Its size is predicted at 11 kDa, but may appear to be 16 kDa due to anomalous migration (3). A 61 aa variant, formed by C-terminal proteolysis, is increased in prostate secretions from patients with benign prostatic hyperplasia (9). A prostate-specific alternate splice form shows a substitution of 41 aa for the C-terminal 78 aa (10). Mature human PSP94 shares 53%, 46%, 43%, and 42% aa identity with porcine, rat, mouse, and chicken PSP94, respectively. Most of the ten primate sequences available show less than 80% aa identity. PSP94 has been proposed as a species barrier protein due to its low evolutionary conservation and abundance in seminal fluid (11).

  • References:
    1. Seidah, N.G. et al. (1984) FEBS Lett. 175:349.
    2. Ulvsback, M. et al. (1989) Biochem. Biophys. Res. Comm. 164:1310.
    3. Weiber, H. et al. (1990) Am. J. Pathol. 137:593.
    4. Thota, A. et al. (2003) J. Cell. Biochem. 88:999.
    5. Wu, D. et al. (1999) J. Cell. Biochem. 76:71.
    6. Udby, L. et al. (2005) Biochem. Biophys. Res. Comm. 333:555.
    7. Reeves, J.R. et al. (2005) Biochem. J. 385:105.
    8. Nam, R.K. et al. (2006) J. Urol. 175:1291.
    9. Xu, K. et al. (2003) Electrophoresis 24:1311.
    10. Xuan, J.W. et al. (1995) Oncogene 11:1041.
    11. Xuan, J.W. et al. (1999) DNA Cell Biol. 18:11.
  • Long Name:
    Prostate Secretory Protein of 94 Amino Acids/Microseminoprotein, beta
  • Entrez Gene IDs:
    4477 (Human); 17695 (Mouse); 29311 (Rat)
  • Alternate Names:
    IGBF; MSMB; MSPB; PIP; PN44; PRPS; PSP57; PSP94
Related Research Areas
Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
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Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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