Human PUM2 Antibody

Catalog # Availability Size / Price Qty
Detection of Human PUM2 by Western Blot.
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Product Details
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Human PUM2 Antibody Summary

Species Reactivity
Detects human PUM2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human PUM1 is observed.
Monoclonal Mouse IgG1 Clone # 740610
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human PUM2
Accession # Q8TB72
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human PUM2 antibody by Western Blot. View Larger

Detection of Human PUM2 by Western Blot. Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PUM2 Monoclonal Antibody (Catalog # MAB7415) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for PUM2 at approximately 115-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PUM2

The Pumilio proteins PUM1 and PUM2 are RNA binding proteins that are repressors of mRNA translation. PUM2 is present in embryonic stem cells and adult germ cells. Both positive and negative influences of PUM2 on proliferation have been reported. Human PUM2 is a 1066 amino acid (aa), 114 kDa (predicted) protein with 6 potential Ser/Thr phosphorylation sites (aa 82, 136, 178, 182, 184, 587, and 672), an acetylation site (aa 672), and a pumilio domain (aa 706-1048). Within aa 103-250, human PUM2 shares 95% aa sequence identity with mouse and rat PUM2. A potential 987 aa isoform lacks aa 574-652.

Long Name
Pumilio 2
Entrez Gene IDs
23369 (Human); 80913 (Mouse); 298874 (Rat)
Alternate Names
FLJ36528; KIAA0235PUMH2PUML2; MGC138251; MGC138253; PUM2; PUMH2; pumilio (Drosphila) homolog 2; pumilio homolog 2 (Drosophila); pumilio homolog 2; Pumilio-2; PUML2

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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