Human PUM2 Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot View Larger

Detection of Human PUM2 by Western Blot. Western blot shows lysates of KG-1 human acute myelogenous leukemia cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human PUM2 Monoclonal Antibody (Catalog # MAB7415) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for PUM2 at approximately 115-130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Preparation and Storage

Background: PUM2

The Pumilio proteins PUM1 and PUM2 are RNA binding proteins that are repressors of mRNA translation. PUM2 is present in embryonic stem cells and adult germ cells. Both positive and negative influences of PUM2 on proliferation have been reported. Human PUM2 is a 1066 amino acid (aa), 114 kDa (predicted) protein with 6 potential Ser/Thr phosphorylation sites (aa 82, 136, 178, 182, 184, 587, and 672), an acetylation site (aa 672), and a pumilio domain (aa 706-1048). Within aa 103-250, human PUM2 shares 95% aa sequence identity with mouse and rat PUM2. A potential 987 aa isoform lacks aa 574-652.

Product Datasheets

Related Research Areas

• Adipose-derived Stem Cell Markers
• Primordial and Mature Germ Cells
• Translation Regulation