Key Product Details

Species Reactivity

Human

Applications

Blockade of Receptor-ligand Interaction, Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2334B
View all PVRIG Primary Antibodies »

Product Specifications

Immunogen

Human embryonic kidney cell line HEK293-derived recombinant human PVRIG
Thr41-Leu172
Accession # Q6DK17

Specificity

Detects human PVRIG in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human PVRIG Antibody

Detection of PVRIG antibody in HEK293 Human Cell Line Transfected with Human PVRIG and eGFP antibody by Flow Cytometry.

Detection of PVRIG in HEK293 Human Cell Line Transfected with Human PVRIG and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human PVRIG or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human PVRIG Monoclonal Antibody (Catalog # MAB93652) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
Nectin-2 Binding to PVRIG Blocked by Human PVRIG Antibody.

Nectin-2 Binding to PVRIG Blocked by Human PVRIG Antibody.

In a functional ELISA, Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) binds to immobilized Recombinant Human PVRIG Fc Chimera (Catalog # 9365-PV) coated at 1 µg/mL (100 µL/well) in a dose-dependent manner (orange line). Binding is blocked (green line) by increasing concentrations of Rabbit Anti-Human PVRIG Monoclonal Antibody (Catalog # MAB93652). At 2.5 µg/mL, this antibody will block >90% of the binding.

Applications for Human PVRIG Antibody

Application
Recommended Usage

Blockade of Receptor-ligand Interaction

In a functional ELISA, 40-240 Ng/mL of this antibody will block 50% of the binding of 2.5 μg/mL of Recombinant Human Nectin-2/CD112 (Catalog # 2229-N2) to immobilized Recombinant Human PVRIG Fc Chimera (Catalog # 9365-PV) coated at 1 µg/mL (100 µL/well). At 2.5 µg/mL, this antibody will block >90% of the binding.

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human PVRIG and eGFP

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PVRIG

Human PVRIG (Poliovirus Receptor Related Immunoglobulin Domain-containing Protein), also known as CD112 receptor (CD112R), is an approximately 34 kDa single transmembrane protein in the poliovirus receptor-like protein (PVR) family (1). It is composed of a single extracellular IgV domain, one transmembrane domain, and a long intracellular domain. The intracellular domain contains two tyrosine residues, one within an ITIM-like motif that is a potential docking site for phosphatases (1). The extracellular domain sequence of human and mouse PVRIG have approximately 65% similarity. The human PVRIG gene is preferentially expressed in lymphocytes, such as T cells and NK cells, but not in monocyte derived dendritic cells (1). PVRIG functions as a cell surface receptor for Nectin-2/CD112, a cell surface protein that is widely expressed on antigen-presenting cells and tumor cells. Disrupting the PVRIG/Nectin-2 interaction enhances human T cell response, suggesting PVRIG is a novel checkpoint for human T cells (1).

References

  1. Zhu, Y., et.al. (2016) J. Exp. Med. 213:167.

Long Name

Poliovirus Receptor Related Immunoglobulin Domain Containing

Alternate Names

C7orf15, CD112R

Entrez Gene IDs

79037 (Human); 102640920 (Mouse); 102127364 (Cynomolgus Monkey)

Gene Symbol

PVRIG

UniProt

Q6DK17

Additional PVRIG Products

Product Documents for Human PVRIG Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human PVRIG Antibody

For research use only

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