Detection of Human Rap2B by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human Rap2B Monoclonal Antibody (Catalog # MAB5710) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Rap2B is a member of the small GTPase superfamily and ras family of proteins. Unlike normal ras proteins that have a nontransforming glutamine residue at aa 61, Rap2B has a threonine residue in that position. This change makes the intrinsic GTPase activity for Rap2B lower, thereby allowing it to exist in the activated state for a longer period of time than normal ras proteins. Aggregation of platelets by thrombin results in the activation of Rap2B in a Ca2+ and PI3 kinase-dependent manner.
Ras-related Protein 2B
Entrez Gene IDs:
5912 (Human); 74012 (Mouse); 170923 (Rat)
MGC20484; Rap2B; RAP2B, member of RAS oncogene family; Ras family small GTP binding protein RAP2B; ras-related protein Rap-2b; small GTP binding protein
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