Human Rap2B Antibody

Catalog # Availability Size / Price Qty
Detection of Human Rap2B by Western Blot.
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Product Details
Supplemental Products

Human Rap2B Antibody Summary

Species Reactivity
Detects human Rap2B in Western blots. No cross-reactivity with recombinant human Rap2A is observed.
Monoclonal Mouse IgG2A Clone # 455517
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant human Rap2B
Accession # P61225
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human Rap2B antibody by Western Blot. View Larger

Detection of Human Rap2B by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 2 µg/mL Mouse Anti-Human Rap2B Monoclonal Antibody (Catalog # MAB5710) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human Rap2A and Rap2B (5 ng/lane) were included. A specific band for Rap2B was detected at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Rap2B

Rap2B is a member of the small GTPase superfamily and ras family of proteins. Unlike normal ras proteins that have a nontransforming glutamine residue at aa 61, Rap2B has a threonine residue in that position. This change makes the intrinsic GTPase activity for Rap2B lower, thereby allowing it to exist in the activated state for a longer period of time than normal ras proteins. Aggregation of platelets by thrombin results in the activation of Rap2B in a Ca2+ and PI3 kinase-dependent manner.

Long Name
Ras-related Protein 2B
Entrez Gene IDs
5912 (Human); 74012 (Mouse); 170923 (Rat)
Alternate Names
MGC20484; Rap2B; RAP2B, member of RAS oncogene family; Ras family small GTP binding protein RAP2B; ras-related protein Rap-2b; small GTP binding protein

Product Datasheets

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Isotype Controls

Mouse IgG2A Isotype Control

Reconstitution Buffers

Secondary Antibodies

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