Human Ras Antibody

Name: Human Ras Antibody
Brand: R&D Systems
SKU: MAB3429
Price: 499 USD
Availability: InStock

R&D Systems | Catalog # MAB3429

Submit a review

Citations (2)

Product Documents

View Available Conjugates

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas
Related Blogs

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG_2B Clone # 342404

View all Ras Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human K-Ras2B
Met1-Met188 (Gln61His)
Accession # P01116-2

Specificity

Detects human Ras (H-Ras, N-Ras, and K-Ras) in Western blots. Does not detect more distantly related Ras superfamily members, including R-Ras, Ral, Rap, and Rheb.

Clonality

Monoclonal

Host

Rat

Isotype

IgG_2B

Scientific Data Images for Human Ras Antibody

Detection of Human Ras by Western Blot.

Western blot shows lysates of K562 human chronic myelo-genous leukemia cell line and Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 5 µg/mL Rat Anti-Human Ras Monoclonal Antibody (Catalog # MAB3429) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). For additional refer-ence, recombinant human H-Ras, N Ras, K-Ras2A and 2B, R-Ras2 and 3, Rap2A and 2B, RalA and B, and Rheb (5 ng/lane) were included. A specific band for Ras was detected at approximately 21 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human Ras Antibody

Application

Recommended Usage

Western Blot

5 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line and Ramos human Burkitt's lymphoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Ras

The Ras proteins (H-Ras, N-Ras, and two splice variants of K-Ras) define a closely related family of 21 kDa GTPases that cycle between GTP-bound active and GDP-bound inactive states at the cytoplasmic face of the plasma membrane. Receptor tyrosine kinases and G-protein coupled receptors activate Ras, and effectors include Raf and PI 3-kinase. Activating mutations of Ras, which impair GTPase activity and stabilize the active state, are found in nearly one-third of all human cancers.