Human Relaxin-2 DuoSet ELISA

R&D Systems | Catalog # DY2804-05

R&D Systems
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Key Product Details

Assay Type

Solid Phase Sandwich ELISA

Assay Range

7.81-500 pg/mL

Sample Type

Cell culture supernates, serum, and plasma
Note: Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet

Reactivity

Human

Human Relaxin-2 DuoSet ELISA Features

  • Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
  • Development protocols are provided to guide further assay optimization
  • Assay can be customized to your specific needs
  • Economical alternative to complete kits
View all Relaxin-2 ELISA Kits »

Product Summary for Human Relaxin-2 DuoSet ELISA

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human Relaxin-2. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Specifications

Assay Format

96-well strip plate (sold separately)

Sample Volume Required

100 µL

Detection Method

Colorimetric ELISA - 450nm (TMB)

Conjugate

Biotin

Specificity

Please see the product datasheet

Label

HRP

Scientific Data Images for Human Relaxin-2 DuoSet ELISA

Human Relaxin-2 ELISA Standard Curve

Human Relaxin-2 ELISA Standard Curve

Kit Contents for Human Relaxin-2 DuoSet ELISA

  • Capture Antibody
  • Detection Antibody
  • Recombinant Standard
  • Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required


PBS:

(Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H2O2) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H2SO4 (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Relaxin-2

Relaxin-2, also called H2 relaxin, is a peptide hormone that plays an important role in female reproduction. It circulates in the blood as a disulfide-linked dimer of alpha and beta chains. Relaxin is best known as a hormone of parturition that promotes growth and softening of the cervix, development of the mammary gland, and has a marked impact on the uterus. In particular, it promotes angiogenesis, inhibits MMP production and activity, and downregulates estrogen receptor-alpha expression. Relaxin-2 confers its activity by binding to leucine-rich guanine nucleotide-binding (G-protein) coupled receptors, LGR7 and LGR8.

Alternate Names

Relaxin2, RLN2, RLXH2

Entrez Gene IDs

6019 (Human)

Gene Symbol

RLN2

Additional Relaxin-2 Products

Product Documents for Human Relaxin-2 DuoSet ELISA

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Relaxin-2 DuoSet ELISA

For research use only

Citations for Human Relaxin-2 DuoSet ELISA

Customer Reviews for Human Relaxin-2 DuoSet ELISA (1)

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  • Human Relaxin-2 DuoSet ELISA
    Name: Jonatan Dereke
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 05/17/2017
    We ran human plasma at a 1:5 dilution for this assay. Due to large variations in patient samples we successfully adjusted the standard curve range to 2000-31.3 pg/ml.
    Human Relaxin-2 DuoSet ELISA DY2804-05

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