Human S100A13 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human S100A13 in ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse S100A13 is observed.
  • Source
    Monoclonal Mouse IgG1 Clone # 854114
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human S100A13
    Ala2-Lys98
    Accession # Q99584
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human S100A13 by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human S100A13 Monoclonal Antibody (Catalog # MAB4327) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for S100A13 at approximately 11-12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.5 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: S100A13

S100A13 is an 11 kDa member of the S100 (soluble in 100% saturated ammonium sulfate) family of vertebrate EF-hand Ca++-binding proteins (1‑3). It is widely expressed as a homodimer with two 98 amino acid (aa) long subunits (2, 3). Human S100A13 shares 83%, 90%, 91%, 87%, 78% and 47% aa identity with mouse, rat, cow, dog, opossum and chicken S100A13, respectively. Like other S100 proteins, S100A13 is small and generally acidic, but contains a basic residue-rich sequence at the C terminus, and two EF hand motifs that bind with Ca++ differing affinities (2‑4). Some S100 proteins, including S100A13, are able to bind the cell surface receptor for advanced glycation end-products (RAGE) (5). Despite lacking a signal sequence, S100A13 plays an important role in Cu++-dependent export of FGF-1 (FGF acidic) and IL-1 alpha from the cell in response to stresses such as heat shock, anoxia and starvation (6‑8). Binding of copper is necessary for formation of a multi-protein complex between S100A13, FGF-1 and p40 synaptotagmin-1 (syt-1) (9, 10). Cu++ ions supplied by S100A13 are thought to oxidize and downregulate the activity of FGF-1 prior to export (10). Calcium influx may also play a similar role in FGF-1 release from neuronal cells (11). S100A13 is composed of four amphiphilic helices that may interact with acidic phospholipid headgroups. With FGF-1 and syt-1, S100A13 likely perturbs the membrane, which allows the S100A13 protein complex to exit the cell (4, 12). S100A13 has been proposed as a marker for angiogenesis in tumors and endometrium, due to its role in stress-induced export of FGF-1 (13, 14). Based on in house studies, S100A13 has also been found to promote neurite outgrowth from rat cortical embryonic neurons (15).

  • References:
    1. Santamaria-Kisiel, L. et al. (2006) Biochem. J. 396:201.  
    2. Wicki, R. et al. (1996) Biochem. Biophys. Res. Commun. 227:594. 
    3. Ridinger, K. et al. (2000) J. Biol. Chem. 275:8686. 
    4. Li, M. et al. (2007) Biochem. Biophys. Res. Commun. 356:616. 
    5. Hsieh, H.-L. et al. (2004) Biochem. Biophys. Res. Commun. 316:949. 
    6. Landriscina, M. et al. (2001) J. Biol. Chem. 276:22544. 
    7. Sivaraja, V. et al. (2006) Biophys. J. 91:1832.
    8. Mandinova, A. et al. (2003) J. Cell Sci. 116:2687.
    9. Prudovsky, I. et al. (2002) J. Cell Biol. 158:201.
    10. Landriscina, M. et al. (2001) J. Biol. Chem. 276:25549.
    11. Matsunaga, H. and H. Ueda (2006) Cell. Mol. Neurobiol. 26:237.
    12. Graziani, I. et al. (2006) Biochem. Biophys. Res. Commun. 349:192.
    13. Landriscina, M. et al. (2006) J. Neurooncol. 80:251.
    14. Hayrabedyan, S. et al. (2005) Reprod. Biol. 5:51.
    15. R&D Sytems (2007) In-house data.
  • Long Name:
    S100 Calcium Binding Protein
  • Entrez Gene IDs:
    6284 (Human); 20196 (Mouse); 295213 (Rat)
  • Alternate Names:
    S100 calcium binding protein A13; S100 calcium-binding protein A13protein S100-A13; S100A13
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