Human Semaphorin 3E Antibody
Human Semaphorin 3E Antibody Summary
Thr25-Ser775 (Arg557Ala and Arg560Ala)
Accession # O15041
Complete Your Research
Complete Your Experiment
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Semaphorin 3E
Semaphorin 3E (Sema3E; previously SemaH) is one of six Class 3 (secreted) semaphorins which in the human share 40-50% amino acid (aa) identity. Class 3 semaphorins are potent chemorepellents that function in axon guidance and/or vascular tip cell guidance during development (1). Sema3E is highly expressed by a subset of motor neurons in developing somites, where it acts as a repulsive cue for PlexinD1-expressing endothelial cells of adjacent intersomitic vessels (2, 3). Crystal structures of semaphorins reveal that the 500 aa N-terminal Sema domain forms a seven-blade b-propeller similar to that found in integrin molecules; 14 conserved cysteine residues and one or more N-glycosylation sites are thought critical for forming the secondary structure (4). C-terminal to the Sema domain, Sema3E has a consensus sequence for furin cleavage which, when used, creates a 61kDa form that does not dimerize and is highly expressed in tumor cell lines with metastatic potential (5, 6). Further C-terminal are a cysteine-knot plexin/semaphorin/integrin (PSI) domain, an Ig-like domain, a cysteine for dimerization and a basic domain containing another furin site. Dimerization and cleavage at the C-terminal site are required for repulsing activity of class 3 semaphorins (7). Human Sema3E shares 90%, 85% and 57% aa identity with mouse, cow and dog Sema3E, respectively. Like other semaphorins, Sema3E signaling is transduced by a transmembrane Plexin dimer, which also has a Sema domain and is coupled to kinase pathways. Unlike other Class 3 semaphorins, Sema3E binds directly to its plexin and does not require interaction with a neuropilin for activity (7). Genetic disruption of either Sema3E or PlexinD1 creates mouse mutants with excessive and disorganized vascular growth and branching, indicating the importance of this ligand-receptor pair for vascular guidance (3, 8).
- Eichmann, A. et al. (2005) Genes Dev. 19:1013.
- Cohen, S. et al. (2005) Eur. J. Neurosci. 21:1767.
- Gu, C. et al. (2005) Science 307:265.
- Gherardi, E. et al. (2004) Curr. Opin. Struct. Biol. 14:669.
- Christensen, C. et al. (1998) Cancer Res. 58:1238.
- Christensen, C. et al. (2005) Cancer Res. 65:6167.
- Adams, R. H. et al. (1997) EMBO J. 16:6077.
- Gitler, A. D. et al. (2004) Developmental Cell 7:107.
No product specific FAQs exist for this product, however you mayView all Antibody FAQs
Reviews for Human Semaphorin 3E Antibody
There are currently no reviews for this product. Be the first to review Human Semaphorin 3E Antibody and earn rewards!
Have you used Human Semaphorin 3E Antibody?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image