Human Serpin B2 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Serpin B2 in direct ELISAs.
  • Source
    Monoclonal Mouse IgG2A Clone # 930119
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human Serpin B2
    Glu2-Ala65, Ala99-Pro415
    Accession # P05120
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.05 µg/mL
    See below
  • Simple Western
    20 µg/mL
    See below
  • Knockout Validated
    2 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Knockout Validated
Western Blot Show Human Serpin B2 Specificity Using Knockout Cell Line. Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Serpin B2 knockout K562 cell line (KO) untreated (-) or treated (+) with 300nM PMA for overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 40 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. HSP60 (Catalog # MAB1800) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Serpin B2 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line (untreated) and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Serpin B2 by Simple WesternTM. Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. Specific bands were detected for Serpin B2 at approximately 45 & 50 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Serpin B2

Serpin B2, also known as Plasminogen Activator Inhibitor 2 (PAI-2), is a 415 amino acid serine-type endopeptidase inhibitor with high expression in blood, vasculature and placenta. Its expression is upregulated in pregnancy and in activated monocytes/macrophages by a wide range of viral, bacterial and parasitic agents. Serpin B2 is upregulated in monocytes from HIV-1 infected patients, and is thought to modulate Th1/Th2 responses. Serpin B2/PAI-2 colocalizes with CHL1 and Vitronectin and mediates neurite outgrowth during post-natal brain development. Inhibition of uPA by SerpinB2 in tumors is associated with a favorable prognosis.

  • Entrez Gene IDs:
    5055 (Human); 18788 (Mouse); 60325 (Rat)
  • Alternate Names:
    HsT1201; Monocyte Arg-serpin; PAI; PAI2; PAI2Urokinase inhibitor; Placental plasminogen activator inhibitor; PLANH2; PLANH2PAI-2; plasminogen activator inhibitor 2; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; Serpin B2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Urokinase Inhibitor
Related Research Areas
Isotype Controls
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Mouse IgG2A Isotype Control

Ctrl MAB003 32  
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Mouse IgG2A Isotype Control

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Mouse IgG2A Isotype Control

Ctrl MAB003R
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Secondary Antibodies
Description Application Cat# Citations Images  

Mouse IgG HRP-conjugated Antibody

WB HAF007 26  
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Mouse F(ab)2 IgG (H+L) PE-conjugated Antibody

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Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugate Antibody

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Donkey Anti-Mouse IgG Biotinylated Antibody

WB BAF018 1
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Mouse IgG HRP-conjugated Antibody

WB HAF018 9  
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Donkey Anti-Mouse IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL008 8
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Mouse F(ab)2 IgG (H+L) APC-conjugated Antibody

Flow F0101B 2  
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Donkey Anti-Mouse IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL009 5
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Mouse IgG Antibody

WB AF007 4
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Goat Anti-Mouse IgG Biotinylated Antibody

WB BAF007 4
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Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0103B 5  
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Mouse/Rabbit IgG VisUCyte HRP Polymer Antibody

IHC VC002  
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Mouse IgG VisUCyte HRP Polymer Antibody

IHC VC001  
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Rat Anti-Mouse IgG2A PE-conjugated Antibody

Flow F0129 1  
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Mouse F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0114  
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Goat Anti-Mouse IgG Fc Affinity Purified Polyclonal Ab

G-202-C 2
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Rat Anti-Mouse IgG2A PerCP-conjugated Antibody

Flow F0131  
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Mouse IgG2A Antibody

Flow MAB0032  
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Rat Anti-Mouse IgG2A APC-conjugated Antibody

Flow F0130  
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