Human Serpin B2 Antibody

Catalog # Availability Size / Price Qty
MAB85501
MAB85501-SP
Western Blot Show Human Serpin B2 Specificity Using Knockout Cell Line.
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Human Serpin B2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin B2 in direct ELISAs.
Source
Monoclonal Mouse IgG2A Clone # 930119
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Serpin B2
Glu2-Ala65, Ala99-Pro415
Accession # P05120
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.05 µg/mL
See below
Simple Western
20 µg/mL
See below
Knockout Validated
2 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Knockout Validated Western Blot Show Human Serpin B2 Specificity Using Knockout Cell Line. View Larger

Western Blot Show Human Serpin B2 Specificity Using Knockout Cell Line. Western blot shows lysates of K562 human chronic myelogenous leukemia parental cell line and Serpin B2 knockout K562 cell line (KO) untreated (-) or treated (+) with 300nM PMA for overnight. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 40 kDa (as indicated) in the parental K562 cell line, but is not detectable in the knockout K562 cell line. HSP60 (Catalog # MAB1800) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western Blot Detection of Human Serpin B2 by Western Blot. View Larger

Detection of Human Serpin B2 by Western Blot. Western blot shows lysates of HEK001 human epidermal keratinocyte cell line (untreated) and U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA. PVDF membrane was probed with 0.05 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Serpin B2 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western Detection of Human Serpin B2 by Simple WesternTM. View Larger

Detection of Human Serpin B2 by Simple WesternTM. Simple Western lane view shows lysates of U937 human histiocytic lymphoma cell line untreated (-) or treated (+) with PMA, loaded at 0.2 mg/mL. Specific bands were detected for Serpin B2 at approximately 45 & 50 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Serpin B2 Monoclonal Antibody (Catalog # MAB85501). This experiment was conducted under reducing conditions and using the
12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin B2

Serpin B2, also known as Plasminogen Activator Inhibitor 2 (PAI-2), is a 415 amino acid serine-type endopeptidase inhibitor with high expression in blood, vasculature and placenta. Its expression is upregulated in pregnancy and in activated monocytes/macrophages by a wide range of viral, bacterial and parasitic agents. Serpin B2 is upregulated in monocytes from HIV-1 infected patients, and is thought to modulate Th1/Th2 responses. Serpin B2/PAI-2 colocalizes with CHL1 and Vitronectin and mediates neurite outgrowth during post-natal brain development. Inhibition of uPA by SerpinB2 in tumors is associated with a favorable prognosis.

Entrez Gene IDs
5055 (Human); 18788 (Mouse); 60325 (Rat)
Alternate Names
HsT1201; Monocyte Arg-serpin; PAI; PAI2; PAI2Urokinase inhibitor; Placental plasminogen activator inhibitor; PLANH2; PLANH2PAI-2; plasminogen activator inhibitor 2; plasminogen activator inhibitor, type II (arginine-serpin); serine (or cysteine) proteinase inhibitor, clade B (ovalbumin), member 2; Serpin B2; serpin peptidase inhibitor, clade B (ovalbumin), member 2; Urokinase Inhibitor

Product Datasheets

FAQs

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Isotype Controls

Mouse IgG2A Isotype Control

Ctrl

Reconstitution Buffers

Secondary Antibodies

Mouse IgG2A Antibody

Mouse IgG2A Antibody

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