Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, Neutralization, Immunoprecipitation

Cited:

Western Blot, Flow Cytometry, ELISA Capture

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Serpin C1/Antithrombin-III Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Serpin C1/Antithrombin-III
His33-Lys464
Accession # P01008

Specificity

Detects human Serpin C1/Antithrombin-III in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse (rm) Serpin C1 is observed and less than 2% cross‑reactivity with recombinant human (rh) Serpin A1, rhSerpin A3, rhSerpin A4, rhSerpin A5, rhSerpin F2, rmSerpin D1 and rhPEDF is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Human Serpin C1/Antithrombin‑III Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Serpin C1/
Antithrombin-III (Catalog # 1267-PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Human Serpin C1/Antithrombin-III (Catalog # 1267-PI)

Neutralization

Measured by its ability to neutralize Recombinant Human Serpin C1 (0.8 µg/mL, Catalog # 1267-PI) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # 1473-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 4.6 µg/mL.

Reviewed Applications

Read 1 review rated 5 using AF1267 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Serpin C1/Antithrombin-III

Serpin C1 is a member of the Serpin superfamily of the serine protease inhibitors (1). It is the principal plasma Serpin of blood clotting proteases and inhibits thrombin as well as several factors such as Xa (2). Similar to Serpins A5 and D1, its thrombin inhibitory activity is enhanced by heparin. Hereditary and acquired Serpin C1 deficiency is the cause of an increased thrombotic tendency in many cases (3). For example, acquired Serpin C1 deficiency is a common condition in sepsis, after major trauma or surgery (4).

References

  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Chuang, Y.-J. et al. (2001) Biochemistry 40:6670.
  3. Vinazzer, H. (1999) Semin. Thromb. Hemost. 25:257.
  4. Risberg, B. (1998) Blood Coagul. Fibrinolysis Suppl. 3:S3.

Alternate Names

Antithrombin-III, ATIII

Entrez Gene IDs

462 (Human); 11905 (Mouse)

Gene Symbol

SERPINC1

UniProt

P01008

Additional Serpin C1/Antithrombin-III Products

Product Documents for Human Serpin C1/Antithrombin‑III Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Serpin C1/Antithrombin‑III Antibody

For research use only

Citations for Human Serpin C1/Antithrombin‑III Antibody

Customer Reviews for Human Serpin C1/Antithrombin‑III Antibody (1)

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  • Human Serpin C1/Antithrombin-III Antibody
    Name: Bryan Thacker
    Application: Flow Cytometry
    Sample Tested: Purified protein
    Species: Human
    Verified Customer | Posted 11/14/2017
    Purified antithrombin bound to cell surface heparan sulfate was detected using 0.2 ug/ml serpin C1/antithrombin antibody and anti-goat-IgG-AlexaFluor488.
    Human Serpin C1/Antithrombin‑III Antibody AF1267

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Associated Pathways

Blood Coagulation Signaling Pathways Blood Coagulation Signaling Pathway Thumbnail