Human Serpin D1/Heparin Cofactor II Antibody

Catalog # Availability Size / Price Qty
AF3198
AF3198-SP
Product Details
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Human Serpin D1/Heparin Cofactor II Antibody Summary

Species Reactivity
Human
Specificity
Detects human Serpin D1/Heparin Cofactor II in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant mouse Serpin D1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Serpin D1/Heparin Cofactor II
Gly20-Ser499
Accession # P05546
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Serpin D1/Heparin Cofactor II (Catalog # 3198-PI)
Immunoprecipitation
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Human Serpin D1/
Heparin Cofactor II (Catalog # 3198-PI), see our available Western blot detection antibodies
Neutralization
Measured by its ability to neutralize Recombinant Human Serpin D1 (0.56 µg/mL, Catalog # 3198-PI) inhibition of Recombinant Human Coagulation Factor II/Thrombin (0.05 µg/mL, Catalog # 1473-SE) cleavage of the fluorogenic peptide substrate Boc-VPR-AMC (100 µM, Catalog # ES011). The Neutralization Dose (ND50) is typically 6.6 µg/mL.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin D1/Heparin Cofactor II

Serpin D1, also known as Heparin Cofactor II, is a member of the Serpin superfamily of the serine protease inhibitors (1). Similar to Serpins A5 and C1, it inhibits thrombin and this activity is enhanced by heparin. Interestingly, a C-terminal His-tagged recombinant Serpin D1 had enhanced heparin effect, which was maintained in a plasma-based thrombin inhibition assay (2). Congenital Serpin D1 deficiency is a potential risk factor for thrombosis (3). The full-length human cDNA was expressed and the purified protein corresponded to the secreted form with anti-thrombin activity.

References
  1. Silverman, G.A. et al. (2001) J. Biol. Chem. 276:33293.
  2. Bauman, S.J. et al. (1999) J. Biol. Chem. 274:34556.
  3. Tollefsen, D.M. (2002) Arch. Pathol. Lab. Med. 126:1394.
Entrez Gene IDs
3053 (Human); 15160 (Mouse)
Alternate Names
HC2; HC2LS2; HCF2; HCF2clade D (heparin cofactor), member 1; HC-II; Heparin Cofactor II; HLS2HCII; leuserpin 2; LS2; Protease inhibitor leuserpin-2; Serpin D1; serpin peptidase inhibitor, clade D (heparin cofactor), member 1

Product Datasheets

FAQs

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ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Serpin D1/Heparin Cofactor II Antibody

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Human Serpin D1/Heparin Cofactor II Antibody
By Anonymous on 12/08/2018
Application: IHC Sample Tested: HUVEC human umbilical vein endothelial cells Species: Human