Detection of Siglec‑7/CD328 in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human NCAM‑1/CD56 PE‑conjugated Monoclonal Antibody (Catalog # FAB2408P) and either (A) Mouse Anti-Human Siglec‑7/CD328 Monoclonal Antibody (Catalog # MAB11381) or (B) Mouse IgG1 Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
(1) (sialic acid binding Ig-like lectins) are I-type (Ig-type) lectins
belonging to the Ig superfamily. They are characterized by an N-terminal
Ig-like V-type domain which mediates sialic acid binding, followed by
varying numbers of Ig-like C2-type domains (1, 2). Eleven human Siglecs
have been cloned and characterized. They are
sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3,
Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11
(1‑4). To date, no Siglec has been shown to recognized any cell surface
ligand other than sialic acids, suggesting that interactions with
glycans containing this carbohydrate are important in mediating the
biological functions of Siglecs. Siglecs 5 to 11 share a high degree of
sequence similarity with CD33/Siglec-3 both in their extracellular and
intracellular regions. They are collectively referred to as CD33-related
Siglecs. One remarkable feature of the CD33-related Siglecs is their
differential expression pattern within the hematopoietic system (2, 3).
This fact, together with the presence of two conserved immunoreceptor
tyrosine-based inhibition motifs (ITIMs) in their cytoplasmic tails,
suggests that CD33-related Siglecs are involved in the regulation of
cellular activation within the immune system. The cDNA of human Siglec-7, also known as adhesion inhibitory
receptor module-1 (AIRM-1) and designated CD328, encodes a 467 amino
acid (aa) polypeptide with a hydrophobic signal peptide, an N-terminal
Ig-like V-type domain, two Ig-like C2-type domains, a transmembrane
region and a cytoplasmic tail (5). Siglec-7 exists as a monomer on the
cell surface and is expressed on natural killer cells, CD8+ T
cells and monocytes (3, 5). It binds equally well to both alpha 2,3- and
alpha 2,6-linked sialic acid (5). The gene encoding Siglec-7 was mapped to
Crocker, P.R. et al. (1998) Glycobiology 8:v.
Crocker, P.R. and A. Varki (2001) Trends Immunol. 22:337.
Crocker, P.R. and A. Varki (2001) Immunology 103:137.
Angata, T. et al. (2002) J. Biol. Chem. 277:24466.
Nicoll, G. et al. (1999) J. Biol. Chem. 274:34089.
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