Human SIRP beta 2 Antibody

Catalog # Availability Size / Price Qty
MAB9998-100
MAB9998-SP
Detection of SIRP beta 2  in HEK293 Human Cell Line Transfected with Human SIRP beta 2 and eGFP by Flow  Cytometry.
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Human SIRP beta 2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human SIRP beta 2 in direct ELISAs.
Source
Monoclonal Mouse IgG1 Clone # 1008709
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
HEK293 human embryonic kidney cell line, HEK293 derived human SIRP beta 2
Gln33-Gly287
Accession # Q5JXA9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of SIRP beta 2  in HEK293 Human Cell Line Transfected with Human SIRP beta 2 and eGFP by Flow  Cytometry. View Larger

Detection of SIRP beta 2 in HEK293 Human Cell Line Transfected with Human SIRP beta 2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) SIRP beta 2 or (B) irrelevant protein, and eGFP were stained with Mouse Anti-Human SIRP beta 2 Monoclonal Antibody (Catalog # MAB9998) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based Mouse IgG2B Isotype Control Antibody staining (Catalog # MAB0041, data not shown). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SIRP beta 2

Signal-regulatory protein beta-2(SIRP-beta-2), is a ~37 kDa monomeric single pass type I membrane glycoprotein. It belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily (1). The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). SIRP-beta-2 contains an N-terminal signal peptide (aa1-32), two extracellular Ig-like domains: a V-type 1 (aa 33-143) and a V-type 2 (aa 157-258) containing three potential N-linked glycosylation sites, a helical transmembrane domain (aa 288-308), and a cytoplasmic domain (aa 309-342) (1). A positively charged residue within the transmembrane domain, in analogy to SIRP-beta-1, is implicated to mediate interaction with the adaptor DAP12 protein, which contains immunoreceptor tyrosine-based activation motifs (ITAMs) (2). Proteins in the SIRP family are typically expressed in immune cells, especially in the myeloid lineages (3). Based on expression patterns, SIRPs are thought to have roles in immune regulation (4). SIRP family members role in innate immunity and host defense has potential significance as a therapeutic target in cancer and inflammation (5, 6). There are currently no known mouse or rat homologs for this protein.

References
  1. van Beek, E.M. et al. (2005) J. Immunol. 175:7781.
  2. Liu, Y. et al. (2005) Journal of Biological Chemistry. 280:36132.
  3. Matozaki, T. et al. (2009) Trends in Cell Biology. 19:72.
  4. Barclay A.N. et al. (2006) Nat Rev Immunol. 6:457.
  5. Barclay A.N. et al. (2014) Annu Rev Immunol. 32:25.
  6. Veillette A. (2018) Trends Immunol. 39:173.
Long Name
Signal-regulatory Protein beta 2
Entrez Gene IDs
284759 (Human)
Alternate Names
dJ776F14.2; Protein Tyrosine Phosphatase Non-Receptor Type Substrate 1-Like 3; Protein Tyrosine Phosphatase Non-Receptor Type Substrate Protein; Protein Tyrosine Phosphatase, Non-Receptor Type 1-Like; Protein Tyrosine Phosphatase, Non-Receptor Type Substrate 1-Like 3; PTPN1L; PTPNS1L3; Signal-Regulatory Protein Beta 2; Signal-Regulatory Protein Beta-2; SIRP beta 2; SIRP-beta-2; SIRPG

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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

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