Human Sirtuin 1/SIRT1 Antibody

Catalog # Availability Size / Price Qty
AF7714
AF7714-SP
Detection of Human Sirtuin 1/SIRT1 by Western Blot.
3 Images
Product Details
FAQs
Supplemental Products
Reviews (1)

Human Sirtuin 1/SIRT1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Sirtuin 1/SIRT1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human SIRT2 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Sirtuin 1/SIRT1
Ala2-Ser747
Accession # Q96EB6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.2 µg/mL
See below
Flow Cytometry
0.25 µg/106 cells
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Knockout Validated
Sirtuin 1/SIRT1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Sirtuin 1/SIRT1 knockout HeLa cell line.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Western Blot Detection of Human Sirtuin 1/SIRT1 by Western Blot. View Larger

Detection of Human Sirtuin 1/SIRT1 by Western Blot. Western blot shows lysates of A172 human glioblastoma cell line, A549 human lung carcinoma cell line, and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection  of Sirtuin 1/SIRT1 in HepG2 Human Cell Line by Flow Cytometry. View Larger

Detection of Sirtuin 1/SIRT1 in HepG2 Human Cell Line by Flow Cytometry. HepG2 human hepatocellular carcinoma cell line was stained with Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714, filled histogram) or isotype control antibody (Catalog # 5-001-A, open histogram), followed by Allophycocyanin-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # F0127). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules.

Knockout Validated Western  Blot Shows Human Sirtuin 1/SIRT1 Specificity by Using Knockout Cell Line. View Larger

Western Blot Shows Human Sirtuin 1/SIRT1 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SIRT1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human Sirtuin 1/SIRT1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7714) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Sirtuin 1/SIRT1 at approximately 120 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sirtuin 1/SIRT1

SIRT1 (SIR2-like protein 1; also NAD-dependent protein deacetylase sirtuin-1 and hSIR2) is a class I member of the sirtuin family of enzymes. Although its predicted MW is 81 kDa, it runs anomalously at 110-120 kDa in SDS-PAGE. It is a widely expressed nuclear protein that participates in the deacetylation of multiple proteins, including p300, p53, LKB1 and histone H1. Functionally, this has the effect of promoting heterochromatin formation, cell survival and resistance to oxidative stress. Metabolically, SIRT1 induces insulin secretion, inhibits glycolysis and suppresses fatty acid synthesis. Human SIRT1 is 747 amino acids (aa) in length. It possesses two NLS's (aa 32-39 and 223-230), an NES (aa 138-145), and a sertuin-type deacetylase domain (aa 241-495) that contains an NAD and Zn binding motif. There are at least 12 utilized Ser/Thr phosphorylation sites, plus two nitrosylated Cys and one acetylated Ala. There are also four potential isoform variants. One is 95 kDa in size and shows a deletion of aa 454-639, a second is 17 kDa in size and contains a 16 aa substitution for aa 149-747, and a third contains an alternative start site at Met296. SIRT1 is also known to undergo proteolysis by cathepsin B at Val533Ser534, generating a fourth, C-terminally truncated 75 kDa isoform. Full-length SIRT1 is suggested to form trimers, while the 17 kDa isoform appears to form dimers. Over aa 2-747, human and mouse SIRT1 share 86% aa sequence identity.

Long Name
Silent Mating Type Information Regulation 2 Homolog
Entrez Gene IDs
23411 (Human); 93759 (Mouse); 309757 (Rat)
Alternate Names
EC 3.5.1; S. cerevisiae, homolog) 1; SIR2alpha; SIR2L1; SIR2-like protein 1; SIRT1; Sirtuin 1

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Human Sirtuin 1/SIRT1 Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
100%
4 Star
0%
3 Star
0%
2 Star
0%
1 Star
0%

Have you used Human Sirtuin 1/SIRT1 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Sirtuin 1/SIRT1 Antibody
By Anonymous on 01/06/2018
Application: WB Sample Tested: HEK293 human embryonic kidney cell line Species: Human