Human Sirtuin 2/SIRT2 Antibody

Catalog # Availability Size / Price Qty
MAB4358
MAB4358-SP
Product Details
FAQs
Supplemental Products
Reviews

Human Sirtuin 2/SIRT2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Sirtuin 2/SIRT2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human SIRT1 is observed.
Source
Monoclonal Mouse IgG1 Clone # 506611
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Sirtuin 2/SIRT2
Ala2-Gln389
Accession # Q8IXJ6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA)
Immunoprecipitation
25 µg/mL
Cell lysates spiked with Recombinant Human Sirtuin 2/SIRT2 (Catalog # 4358-DA), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sirtuin 2/SIRT2

Sirtuin 2, encoded by the SIRT2 gene, is also known as SIR2 (silent information regulator 2)-like protein 2. It is a nicotinamide adenine dinucleotide (NAD)-dependent histone/protein deacetylase (1, 2). The SIR2 family of enzymes is classified as class III histone deacetylases (HDACs) and has been implicated in many cellular processes that include histone deacetylation, gene silencing, chromosomal stability, and aging (3, 4). Unlike class I and class II HDACs, the enzymatic activity of class III HDACs is NAD dependent and insensitive to HDAC inhibitor trichostatin A (5).

References
  1. Frye, R.A. (1999) Biochem. Biophys. Res. Commun. 260:273.
  2. North, B.J. et al. (2003) Mol. Cell. 11:437.
  3. North, B.J. and Verdin, E. (2004) Genome Biol. 5:224.
  4. Marmorstein, R. (2004) Biochem. Soc. Trans. 32:904.
  5. Gray, S.G. and Ekstrom, T.J. (2001) Exp. Cell Res. 262:75.  
Long Name
Silent Mating Type Information Regulation 2 Homolog
Entrez Gene IDs
22933 (Human); 64383 (Mouse); 361532 (Rat)
Alternate Names
EC 3.5.1; EC 3.5.1.-; FLJ35621; FLJ37491; S.cerevisiae, homolog) 2; SIR2L2; SIRT2; Sirtuin 2; sirtuin type 2

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

ExactaChIP Chromatin Immunoprecipitation Kits

Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Sirtuin 2/SIRT2 Antibody

There are currently no reviews for this product. Be the first to review Human Sirtuin 2/SIRT2 Antibody and earn rewards!

Have you used Human Sirtuin 2/SIRT2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review