Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all SIT1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human SIT1
Gln65-Ser196
Accession # Q9Y3P8

Specificity

Detects human SIT1 in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Human SIT1 Antibody

Application
Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human SIT1

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIT1

SIT1 (SHP-2-interacting transmembrane adapter 1; also signaling threshold-regulating transmembrane adapter 1) is a 35‑40 kDa type I transmembrane glycoprotein that belongs to a diverse group of transmembrane adapter proteins. It is expressed on thymocytes, plasma cells and T cells. When engaged, SIT1 downregulates TCR signaling. Mature human SIT1 is 172 amino acids (aa) in length. It contains a short extracellular domain (aa 25‑40) that undergoes homodimerization and an extensive intracellular region (aa 62‑196) that contains three key phosphorylation sites (Tyr90/169/188). Over aa 65‑196, human SIT1 is 86% and 80% aa identical to canine and mouse SIT1, respectively.

Long Name

Signaling Threshold Regulating Transmembrane Adaptor 1

Alternate Names

gp30/40

Entrez Gene IDs

27240 (Human)

Gene Symbol

SIT1

UniProt

Q9Y3P8

Additional SIT1 Products

Product Documents for Human SIT1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human SIT1 Antibody

For research use only

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FAQs

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